School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong 255049, China.
Sci Total Environ. 2022 Oct 20;844:157184. doi: 10.1016/j.scitotenv.2022.157184. Epub 2022 Jul 6.
An ultrasensitive electrochemiluminescence (ECL) aptasensor for detection of profenofos was constructed by the reducibility and chemiluminescence property of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). ABEI was used to reduce silver nitrate (AgNO) to silver nanoparticles (AgNPs), which could be adsorbed on the lattice of graphene oxide (GO) to form ABEI-AgNPs-GO complex. This compound could achieve excellent luminescence. The aptamer (Apt) modified (5') by sulfhydryl groups could be immobilized on AgNPs to capture profenofos. When profenofos was present, the ECL signal of the aptasensor would be weakened. To further demonstrate the successful construction of the aptasensor, cyclic voltammetry tests were performed on an electrochemical workstation and an ECL analyzer, respectively. The standard curve and specificity experiment both showed that the sensor had the advantages of low limit of detection (LOD) and good specificity. Under the optimal conditions, the aptasensor had a good linear response for profenofos in the range of 1 × 10-1 × 10 ng/mL. It also had a LOD of 6.7 × 10 ng/mL and a correlation coefficient (R) of 0.9991. The aptasensor had been successfully applied to the detection of profenofos in vegetables. The recovery range of the proposed ECL aptasensor was 98 % ~ 107.4 %.
一种超灵敏的电化学发光（ECL）适体传感器，通过 N-(氨基丁基)-N-(乙基异鲁米诺)（ABEI）的还原性和化学发光性质构建。ABEI 用于还原硝酸银（AgNO）为纳米银颗粒（AgNPs），这些颗粒可以被吸附在氧化石墨烯（GO）的晶格上，形成 ABEI-AgNPs-GO 复合物。这种复合物可以实现出色的发光效果。巯基修饰的适体（Apt）（5'端）可以固定在 AgNPs 上，用于捕获丙溴磷。当存在丙溴磷时，适体传感器的 ECL 信号会减弱。为了进一步证明适体传感器的成功构建，分别在电化学工作站和 ECL 分析仪上进行了循环伏安测试。标准曲线和特异性实验均表明，该传感器具有低检测限（LOD）和良好特异性的优点。在最佳条件下，该适体传感器对丙溴磷在 1×10-1×10 ng/mL 范围内具有良好的线性响应。其 LOD 为 6.7×10 ng/mL，相关系数（R）为 0.9991。该适体传感器已成功应用于蔬菜中丙溴磷的检测。所提出的 ECL 适体传感器的回收率范围为 98%~107.4%。
