Khurana N K, Wales R G
School of Veterinary Studies, Murdoch University, W.A.
Aust J Biol Sci. 1987;40(4):389-95. doi: 10.1071/bi9870389.
Coculture of mouse morulae/early blastocysts with isolated endometrial epithelial cells reduced incorporation of glucose carbon into embryonic glycogen but had no significant effect on incorporation into other internal carbon pools during a 5-h culture in serum-supplemented Dulbecco's modification of Eagle's minimum essential medium. Turnover of glycogen pools during 24-h chase culture of pulse-labelled embryos was unaffected by the presence of uterine epithelial cells recovered from day-4 pregnant or non-pregnant mice. However, significantly more label was retained in non-glycogen macromolecules during chase in the presence of endometrium recovered from non-pregnant than from pregnant uteri.
在添加血清的杜尔贝科改良伊格尔最低限度基本培养基中进行5小时培养时，将小鼠桑椹胚/早期囊胚与分离的子宫内膜上皮细胞共培养，会减少葡萄糖碳掺入胚胎糖原，但对掺入其他内部碳库没有显著影响。脉冲标记胚胎在24小时追踪培养期间糖原库的周转不受从第4天怀孕或未怀孕小鼠回收的子宫上皮细胞的影响。然而，在追踪过程中，与从怀孕子宫回收的子宫内膜相比，从未怀孕子宫回收的子宫内膜存在时，非糖原大分子中保留的标记明显更多。
