Khurana N K, Wales R G
J Reprod Fertil. 1987 Jan;79(1):267-73. doi: 10.1530/jrf.0.0790267.
The addition of progesterone (10(-7) to 10(-5) M) and/or oestradiol (10(-10) M) during 24-h chase culture of pulse-labelled morulae-early blastocysts did not affect the degradation of radiolabelled glycogen or other biochemical fractions. The presence of a high concentration of progesterone (10(-5) M) during 5-h pulse culture significantly inhibited incorporation of substrate carbon from [U-14C]glucose into both the acid-soluble and acid-insoluble glycogen fractions, but had no effect on non-glycogen fractions. Catabolic utilization of glucose as estimated by the rate of carbon dioxide and lactate production was not affected by the presence of progesterone (10(-7) to 10(-5) M), oestradiol (10(-10) to 10(-8) M) or a combination of both. The results indicate that ovarian steroids at expected physiological concentrations do not directly influence embryonic energy metabolism.
在对脉冲标记的桑椹胚-早期囊胚进行24小时追踪培养期间，添加孕酮（10⁻⁷至10⁻⁵M）和/或雌二醇（10⁻¹⁰M）不会影响放射性标记糖原或其他生化组分的降解。在5小时脉冲培养期间存在高浓度孕酮（10⁻⁵M）会显著抑制[U-¹⁴C]葡萄糖的底物碳掺入酸溶性和酸不溶性糖原组分中，但对非糖原组分没有影响。通过二氧化碳和乳酸产生速率估算的葡萄糖分解代谢利用不受孕酮（10⁻⁷至10⁻⁵M）、雌二醇（10⁻¹⁰至10⁻⁸M）或两者组合的影响。结果表明，预期生理浓度的卵巢类固醇不会直接影响胚胎能量代谢。
