Brignone J A, Ricci C R, de Brignone C M, Labonia N A, Stoppani A O
Cátedra de Química Biológica, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Biochem Int. 1987 Apr;14(4):605-16.
Liver D-3-hydroxybutyrate dehydrogenase (OHBD) is subjected to estrogen modulation. Estrogen action was demonstrated by (a) the lesser activity of liver OHBD in female rats, as compared with their male counterparts; (b) the increase of OHBD activity after ovariectomy of sexually mature rats; (c) the decrease of OHBD activity after treatment of gonadectomized or normal rats with 17 beta-estradiol or with artificial estrogens; (d) the decrease of OHBD activity in female rats during sexual development; (e) the effects of tamoxifen on the enzyme activity. The kinetics of OHBD reaction using liver mitochondria from estrogen-treated rats showed a 50% decrease of Vmax, as compared with the control value, in contrast to the other parameters which did not vary. These results, taken together with the effect of estrogens on liver mitochondrial phospholipids, point to a decreased content of OHBD in liver mitochondria from estrogen-treated rats. In contrast to OHBD, succinate dehydrogenase and cytochrome oxidase activities, mitochondrial protein synthesis and L-malate + L-glutamate oxidation by coupled liver mitochondria either increased or were not affected by estrogens. Kidney and heart OHBD were affected by ovariectomy and estrogens like the liver enzyme, though to a lesser degree.
肝脏D-3-羟基丁酸脱氢酶（OHBD）受雌激素调节。雌激素的作用通过以下几点得到证明：（a）与雄性大鼠相比，雌性大鼠肝脏OHBD的活性较低；（b）性成熟大鼠卵巢切除后OHBD活性增加；（c）用17β-雌二醇或人工雌激素处理去势或正常大鼠后OHBD活性降低；（d）雌性大鼠在性发育期间OHBD活性降低；（e）他莫昔芬对酶活性的影响。与对照值相比，使用经雌激素处理大鼠的肝脏线粒体进行OHBD反应的动力学显示Vmax降低了50%，而其他参数没有变化。这些结果与雌激素对肝脏线粒体磷脂的作用一起表明，经雌激素处理大鼠的肝脏线粒体中OHBD含量降低。与OHBD不同，琥珀酸脱氢酶和细胞色素氧化酶活性、线粒体蛋白质合成以及肝脏线粒体偶联氧化L-苹果酸+L-谷氨酸的过程要么增加，要么不受雌激素影响。肾脏和心脏的OHBD与肝脏酶一样受到卵巢切除和雌激素的影响，不过程度较轻。
