疣孢漆斑菌素生物合成步骤的时间分离

Temporal separation of steps in the biosynthesis of verruculogen.

作者信息

Mantle P G, Shipston N F

机构信息

Biochemistry Department, Imperial College, London, U.K.

出版信息

Biochem Int. 1987 Jun;14(6):1115-20.

PMID:3453096

Abstract

A mixture of 2[3H] mevalonate and U[14C] proline, given to Penicillium simplicissimum on days 2, 3, 4, 5 or 6 of stationary liquid culture, gave rise to radiolabelled verruculogen in which the ratio of 3H to 14C measured on day 8 increased from 2.0:1 to 9.5:1 over the period of administration of radiolabelled precursors. This suggests temporal separation of the formation of the diketopiperazine moiety and its subsequent prenylation. The putative precursor diketopiperazine, cyclo-L-tryptophanyl-L-proline, was isolated from 21 day culture broth.

摘要

在静止液体培养的第2、3、4、5或6天，将2[³H]甲羟戊酸和U[¹⁴C]脯氨酸的混合物给予简单青霉，产生了放射性标记的疣孢菌素，在给予放射性标记前体的期间，第8天测得的³H与¹⁴C的比率从2.0:1增加到9.5:1。这表明二酮哌嗪部分的形成与其随后的异戊烯基化在时间上是分开的。从21天的培养液中分离出了假定的前体二酮哌嗪，即环-L-色氨酰-L-脯氨酸。