Rowland R R, Chukwuocha R, Tokuda S
Department of Microbiology, School of Medicine, University of New Mexico, Albuquerque 87131.
Brain Behav Immun. 1987 Dec;1(4):342-8. doi: 10.1016/0889-1591(87)90037-7.
The in vitro priming of mouse spleen cultures with sheep erythrocytes (SE) was used to study the modulation of immune function by met-enkephalin (MENK). In these studies, suboptimal, optimal, and supraoptimal concentrations of SE were used to manipulate the plaque-forming cell (PFC) responses of cultured spleen cells. MENK, at a concentration of 10(-7) M, was able to abolish the high antigen dose-induced suppression of the PFC response, but was unable to increase the PFC response of cultures treated with suboptimal doses of antigen. On rare occasions when the supraoptimal dose of antigen did not suppress the immune response, the addition of 10(-7) M MENK to the culture medium suppressed the PFC response. Naloxone was unable to block the effect of MENK. These results indicate that the nature of the immune response must be taken into consideration when evaluating the effect of opioid peptides on immune function. We propose that MENK possesses a dual modulatory role, with the abilities to suppress a strong immune response and reverse high antigen-induced immunosuppression.
用绵羊红细胞（SE）对小鼠脾细胞培养物进行体外致敏，以研究甲硫氨酸脑啡肽（MENK）对免疫功能的调节作用。在这些研究中，使用次优、最优和超优浓度的SE来调控培养脾细胞的空斑形成细胞（PFC）反应。浓度为10^(-7) M的MENK能够消除高抗原剂量诱导的PFC反应抑制，但不能增强用次优剂量抗原处理的培养物的PFC反应。在极少数情况下，当超优剂量的抗原未抑制免疫反应时，向培养基中添加10^(-7) M的MENK会抑制PFC反应。纳洛酮无法阻断MENK的作用。这些结果表明，在评估阿片肽对免疫功能的影响时，必须考虑免疫反应的性质。我们提出，MENK具有双重调节作用，既能抑制强烈的免疫反应，又能逆转高抗原诱导的免疫抑制。
