Lida Y
Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Japan.
Comput Appl Biosci. 1987 Jun;3(2):93-8. doi: 10.1093/bioinformatics/3.2.93.
The signals which direct excision of introns from mRNA precursors in higher eukaryotes' genes are not well understood. Although a consensus sequence, CAAG/GTAGAGT, has been proposed with the 5' splice site, actual 5' splice site sequences differ from it to a greater or lesser degree. In order to study such a signal more quantitatively, nucleotide sequences were transformed into categorical data, and multivariate statistical analysis was applied to such a system. Categorical weights on the variables were estimated in such a way that the two classes of 5' splice site sequences and sequences other than 5' splice site might be discriminated most distinctly. The 5' splice site signals were then characterized in terms of those statistical results.
高等真核生物基因中指导从mRNA前体切除内含子的信号尚未得到充分理解。尽管已提出5'剪接位点的共有序列CAAG/GTAGAGT，但实际的5'剪接位点序列与之或多或少存在差异。为了更定量地研究此类信号，将核苷酸序列转化为分类数据，并对该系统应用多元统计分析。以最清晰地区分5'剪接位点序列的两类和非5'剪接位点序列的方式估计变量的分类权重。然后根据这些统计结果对5'剪接位点信号进行表征。
