Zhang Zhongyuan, Guan Bao, Li Yifan, He Qun, Li Xuesong, Zhou Liqun
Department of Urology, Peking University First Hospital, Beijing, China.
Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
Transl Androl Urol. 2021 Aug;10(8):3348-3357. doi: 10.21037/tau-21-371.
This study aims to investigate the level of cAMP response element-binding protein 1 (phospho S133) (p-CREB1) protein in clear cell renal cell carcinoma (ccRCC) and evaluates its prognosis significance.
Immunohistochemistry (IHC) method was performed to detect p-CREB1 staining in 233 ccRCC patients. Three or more high-power fields per tissue section were equally captured by a Leica DMRXA microphotographic system, and average staining intensity (optical density, OD) was analyzed by Leica Qwin Standard V2.6 system. Univariate and multivariate Cox proportional regression model was performed to assess the correlation of p-CREB1 staining and clinical outcomes.
IHC proved that the level of p-CREB1 protein was significantly higher in tumor tissues than in adjacent normal tissues, and gradually increased from normal to tumor sections. On the basis of the receiver operating characteristic curve, patients were divided into low p-CREB1 staining (OD ≤0.28) and high p-CREB1 staining subgroup (OD >0.28) according to p-CREB1 protein staining intensity of tumor cells. Multivariate analyses showed that high p-CREB1staining was an independent risk factor for cancer-specific free survival, overall survival and progression-free survival.
p-CREB1 protein is an independent prognostic biomarker for ccRCC patients.
本研究旨在探讨透明细胞肾细胞癌（ccRCC）中cAMP反应元件结合蛋白1（磷酸化S133）（p-CREB1）蛋白的水平，并评估其预后意义。
采用免疫组织化学（IHC）方法检测233例ccRCC患者的p-CREB1染色情况。用徕卡DMRXA显微摄影系统在每个组织切片上均匀采集三个或更多高倍视野，并用徕卡Qwin Standard V2.6系统分析平均染色强度（光密度，OD）。采用单因素和多因素Cox比例回归模型评估p-CREB1染色与临床结局的相关性。
免疫组织化学证实，肿瘤组织中p-CREB1蛋白水平显著高于相邻正常组织，且从正常组织到肿瘤组织切片逐渐升高。根据肿瘤细胞的p-CREB1蛋白染色强度，基于受试者工作特征曲线将患者分为低p-CREB1染色（OD≤0.28）和高p-CREB1染色亚组（OD>0.28）。多因素分析表明，高p-CREB1染色是癌症特异性无病生存、总生存和无进展生存的独立危险因素。
p-CREB1蛋白是ccRCC患者的独立预后生物标志物。
