College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; State Key Laboratory of Food Nutrition and Safety, Tianjin 300457, China.
Int J Biol Macromol. 2021 Nov 1;190:887-893. doi: 10.1016/j.ijbiomac.2021.09.055. Epub 2021 Sep 15.
Carboxymethylated cellulose nanofibrils (CNF) with different carboxyl contents (0, 0.36, 0.72 and 1.24 mmol/g) were prepared and characterized via morphology, diameter distribution, zeta potential, structural features, rheological properties, suspension stability, and thermal properties. The results of toxicological studies of ingested CNF via in vitro and in vivo models were present. In vitro studies used an epithelial-like cell line (Caco-2) to assess the effects of a 24 h incubation with CNF, in which no significant cytotoxicity was observed. In vivo studies were evaluated in mice gavage once per day for 8 weeks with 1% or 3.5% w/w suspension of CNF in water. Blood and serum were collected for analysis. No significant differences in hematology, and serum markers were observed between controls and mice given CNF suspensions. Weight, food intake and feces were recorded for growing development and nutrient retention in feces was measured for investigation of functional properties of CNFs. Mice given CNF suspensions gained a significant increment in fecal fat but a reduction in food intake and weight compared to controls. These findings suggested that CNFs are non-toxic and have potentials in behaving as food additives or supplements to reduce caloric intake.
羧甲基化纤维素纳米纤维（CNF）具有不同的羧基含量（0、0.36、0.72 和 1.24mmol/g），通过形态、直径分布、zeta 电位、结构特征、流变性能、悬浮稳定性和热性能进行了表征。还介绍了通过体外和体内模型摄入 CNF 的毒理学研究结果。体外研究使用上皮样细胞系（Caco-2）评估与 CNF 孵育 24 小时的影响，未观察到明显的细胞毒性。体内研究在小鼠中进行，每天灌胃一次，连续 8 周，用 1%或 3.5%w/w 的 CNF 水混悬液。采集血液和血清进行分析。与给予 CNF 混悬液的小鼠相比，对照组的血液学和血清标志物无显著差异。记录体重、食物摄入量和粪便，以研究生长发育过程中的营养保留情况，并测量粪便中 CNF 的功能特性。与对照组相比，给予 CNF 混悬液的小鼠粪便中的脂肪显著增加，但食物摄入量和体重减少。这些发现表明 CNF 无毒，具有作为食品添加剂或补充剂的潜力，可减少热量摄入。
