Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, 550025, China; Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province, Guizhou University, Guiyang, Guizhou, 550025, China; College of Forestry, Guizhou University, Guiyang, 550025, China.
Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, 550025, China; Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou Province, Guizhou University, Guiyang, Guizhou, 550025, China; College of Forestry, Guizhou University, Guiyang, 550025, China.
Fungal Biol. 2021 Oct;125(10):785-795. doi: 10.1016/j.funbio.2021.05.001. Epub 2021 May 13.
Fungi play key roles in forest ecosystems and help to shape the forest's diverse functions. However, little is known about the diversity of phyllospheric fungi or their possible relationships with fungal communities residing in different micro-environments of Pinus massoniana forests. We investigated seven different sample types: mature needles (NM), dead needles (ND), needles falling as litter (L), fermenting needles (F), humus (H), top soil (0-20 cm) (TS), and secondary soil (20-40 cm) (SS). These seven fungal communities were examined and compared with ITS amplicons using a high-throughput sequencing technique. A total of 1213 fungal operational taxonomic units (OTUs) were obtained at a 97% sequence similarity level. Distinct fungal communities were associated with different sample types. A greater number of OTUs were present in both NM and F samples than those shared by both NM and TS samples, indicating that phyllospheric fungi may play crucial roles in litter decomposition. Sixty OTUs (the core microbiome) were found in all sample types, and they may probably play different ecological roles in different sample types. These findings extend our knowledge of the fungal diversity of the phyllosphere and its possible interactions with fungal communities found in distinct forest micro-habitats.
真菌在森林生态系统中发挥着关键作用，有助于塑造森林的多样化功能。然而，人们对叶际真菌的多样性及其与不同微生境中栖息的真菌群落的可能关系知之甚少。我们研究了 7 种不同的样本类型：成熟针叶（NM）、凋落针叶（ND）、凋落针叶（L）、发酵针叶（F）、腐殖质（H）、表土（0-20cm）（TS）和次层土（20-40cm）（SS）。使用高通量测序技术对这 7 个真菌群落进行了检测和 ITS 扩增子比较。在 97%序列相似性水平下共获得了 1213 个真菌操作分类单元（OTUs）。不同的样本类型与不同的真菌群落相关。NM 和 F 样本中存在的 OTU 数量明显多于 NM 和 TS 样本中共同存在的 OTU 数量，这表明叶际真菌可能在凋落叶分解中发挥关键作用。在所有样本类型中都发现了 60 个 OTUs（核心微生物组），它们可能在不同的样本类型中发挥不同的生态作用。这些发现扩展了我们对叶际真菌多样性及其与不同森林小生境中发现的真菌群落相互作用的认识。
