College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi, 712100, China; Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China.
Agriculture and Rural Bureau of Jianshi County, Jianshi 445300, Hubei, China.
Food Chem. 2022 Feb 15;370:130982. doi: 10.1016/j.foodchem.2021.130982. Epub 2021 Aug 30.
Although the Se concentration and recovery efficiency of soybean seeds treated with selenate were ∼ 1.8 times those of the selenite treatment, the Se was mainly in the organic form of selenomethionine (>90% of total Se) irrespective of the Se source. The Se concentrations of soybean protein isolate (SPI) and glycinin (11S) were 29.1%-38.6% higher than those of soybean protein concentrate (SPC) and β-conglycinin (7S) in Se-enriched soybeans, with selenomethionine accounting for > 80% of the Se in all proteins. The content of sulfur-containing methionine in SPI and 11S markedly decreased in Se-enriched soybeans compared with the control. No significant effect of Se was observed on protein content, subunit composition, secondary structure, micromorphology, or functionality. Foliar spray of selenate provides an economical and efficient way to produce Se-enriched soybeans without affecting protein structure and functionality, where SPI and 11S display a high ability to enrich Se (mainly selenomethionine).
尽管用亚硒酸盐处理的大豆种子的硒浓度和回收率约为亚硒酸盐处理的 1.8 倍，但无论硒源如何，硒主要以硒代蛋氨酸的有机形式存在（>总硒的 90%）。富硒大豆中的大豆分离蛋白（SPI）和球蛋白（11S）的硒浓度比大豆浓缩蛋白（SPC）和β-伴球蛋白（7S）高 29.1%-38.6%，所有蛋白质中的硒> 80%来自硒代蛋氨酸。与对照相比，富硒大豆中 SPI 和 11S 中的含硫蛋氨酸含量明显下降。硒对蛋白质含量、亚基组成、二级结构、微观形态或功能没有显著影响。叶面喷施亚硒酸盐是一种经济有效的生产富硒大豆的方法，不会影响蛋白质的结构和功能，其中 SPI 和 11S 具有很高的富集硒（主要是硒代蛋氨酸）的能力。
