Xiong Yuzhou, Xiang Xumin, Xiao Chunmei, Zhang Na, Cheng Hua, Rao Shen, Cheng Shuiyuan, Li Li
School of Modern Industry for Selenium Science and Engineering, National R&D Center for Se-Rich Agricultural Products Processing Technology, Wuhan Polytechnic University, Wuhan 430023, China.
Plants (Basel). 2023 Feb 9;12(4):789. doi: 10.3390/plants12040789.
Selenium (Se) is an essential element for mammals, and its deficiency in the diet is a global problem. Agronomic biofortification through exogenous Se provides a valuable strategy to enhance human Se intake. Selenium nanoparticles (SeNPs) have been regarded to be higher bioavailability and less toxicity in comparison with selenite and selenate. Still, little has been known about the mechanism of their metabolism in plants. Soybean ( L.) can enrich Se, providing an ideal carrier for Se biofortification. In this study, soybean sprouts were treated with SeNPs, and a combination of next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing was applied to clarify the underlying molecular mechanism of SeNPs metabolism. A total of 74,662 nonredundant transcripts were obtained, and 2109 transcription factors, 9687 alternative splice events, and 3309 long non-coding RNAs (lncRNAs) were predicted, respectively. KEGG enrichment analysis of the DEGs revealed that metabolic pathways, biosynthesis of secondary metabolites, and peroxisome were most enriched both in roots and leaves after exposure to SeNPs. A total of 117 transcripts were identified to be putatively involved in SeNPs transport and biotransformation in soybean. The top six hub genes and their closely coexpressed Se metabolism-related genes, such as (), (), and (), were screened by WGCNA and identified to play crucial roles in SeNPs accumulation and tolerance in soybean. Finally, a putative metabolism pathway of SeNPs in soybean was proposed. These findings have provided a theoretical foundation for future elucidation of the mechanism of SeNPs metabolism in plants.
硒（Se）是哺乳动物必需的元素，其在饮食中的缺乏是一个全球性问题。通过外源硒进行农艺生物强化是提高人类硒摄入量的一项有价值的策略。与亚硒酸盐和硒酸盐相比，硒纳米颗粒（SeNPs）被认为具有更高的生物利用度和更低的毒性。然而，人们对它们在植物中的代谢机制仍知之甚少。大豆（Glycine max (L.)）能够富集硒，为硒生物强化提供了理想的载体。在本研究中，用SeNPs处理大豆芽，并应用下一代测序（NGS）和单分子实时（SMRT）测序相结合的方法来阐明SeNPs代谢的潜在分子机制。共获得74,662条非冗余转录本，分别预测出2109个转录因子、9687个可变剪接事件和3309个长链非编码RNA（lncRNAs）。对差异表达基因（DEGs）的KEGG富集分析表明，暴露于SeNPs后，根和叶中代谢途径、次生代谢物的生物合成和过氧化物酶体的富集程度最高。共鉴定出117个转录本可能参与大豆中SeNPs的转运和生物转化。通过加权基因共表达网络分析（WGCNA）筛选出前六个枢纽基因及其紧密共表达的硒代谢相关基因，如GmNIP2;1、GmABCC1和GmGSH1，并确定它们在大豆SeNPs积累和耐受性中起关键作用。最后，提出了大豆中SeNPs的一种假定代谢途径。这些发现为今后阐明植物中SeNPs代谢机制提供了理论基础。
