Filez L, Stalmans W, Penninckx F, Kerremans R, Geboes K
Department of Abdominal Surgery, Faculty of Medicine, Catholic University of Leuven, Belgium.
Biosci Rep. 1987 Dec;7(12):917-23. doi: 10.1007/BF01122124.
Lactate dehydrogenase has been measured in the small-intestinal mucosa in order to assess its value as a marker for the effects of ischemia and of reperfusion. The decrease in specific activity of the enzyme illustrates the deleterious effect of reperfusion on the quality of the remaining epithelial cells. However, this parameter fails to detect the loss of epithelial cells, which is the major event during ischemia as well as during reperfusion. In contrast, the expression of enzyme activity per g protein of the underlying intestinal muscle allowed us, in addition, to assess quantitatively the loss of epithelial cells, in good agreement with the histological data.
为了评估乳酸脱氢酶作为缺血和再灌注影响标志物的价值，已对小肠黏膜中的乳酸脱氢酶进行了测量。该酶比活性的降低说明了再灌注对剩余上皮细胞质量的有害影响。然而，该参数未能检测到上皮细胞的丢失，而上皮细胞丢失是缺血和再灌注期间的主要事件。相比之下，根据每克肠道肌肉蛋白的酶活性表达，我们还能够定量评估上皮细胞的丢失情况，这与组织学数据高度吻合。
