Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering, CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Bioresour Technol. 2021 Dec;342:125854. doi: 10.1016/j.biortech.2021.125854. Epub 2021 Sep 9.
The role of poised (negative/positive) potential (0.2/0.4/0.6/0.8 V vs Ag/AgCl at anode) was studied in electrofermentation system (EF) to understand the metabolic flux of Bacillus subtilis with pyruvate as a carbon source. The relative expression of genes encoding pyruvate dehydrogenase (pdhA), lactate dehydrogenase (lctE), acetate kinase (ackA), pyruvate carboxylase (pycA), adenylosuccinate lyase (purB), acylCoA dehydrogenase (acdA) and NADH dehydrogenase (ndh) allowed evaluation of metabolic changes in correlation to product formation and bioelectrochemical analysis. In comparison to control, poised circumstances showed marked influence on product profile with up-regulation of key enzymes involved in pyruvate metabolism. EF poised with - 0.8 V and -0.6 V enhanced bio-hydrogen production by 6 folds and 4 folds respectively. Concomitantly, -0.8 V resulted in maximum ethanol and acetic acid production whilst, -0.6 V and + 0.6 V resulted in maximum lactic acid and succinic acid production respectively. The transcripts for genes associated synthesis were upregulated in the respected poised reactors.
在电发酵系统 (EF) 中研究了（负/正） poised 电位（相对于阳极的 0.2/0.4/0.6/0.8 V 与 Ag/AgCl），以了解枯草芽孢杆菌以丙酮酸作为碳源的代谢通量。与对照相比，（负/正） poised 状态下与产物形成和生物电化学分析相关的基因编码丙酮酸脱氢酶（pdhA）、乳酸脱氢酶（lctE）、乙酰激酶（ackA）、丙酮酸羧化酶（pycA）、腺嘌呤琥珀酸裂解酶（purB）、酰基辅酶 A 脱氢酶（acdA）和 NADH 脱氢酶（ndh）的相对表达允许评估代谢变化。与对照相比， poised 状态对产物分布产生了明显的影响，上调了与丙酮酸代谢相关的关键酶。与对照相比，-0.8 V 和-0.6 V 的 EF 分别使生物氢气产量提高了 6 倍和 4 倍。同时，-0.8 V 导致最大的乙醇和乙酸产量，而-0.6 V 和+0.6 V 导致最大的乳酸和琥珀酸产量。与合成相关的基因的转录物在相应的 poised 反应器中上调。
