Metabolic flux of Bacillus subtilis under poised potential in electrofermentation system: Gene expression vs product formation.

The role of poised (negative/positive) potential (0.2/0.4/0.6/0.8 V vs Ag/AgCl at anode) was studied in electrofermentation system (EF) to understand the metabolic flux of Bacillus subtilis with pyruvate as a carbon source. The relative expression of genes encoding pyruvate dehydrogenase (pdhA), lactate dehydrogenase (lctE), acetate kinase (ackA), pyruvate carboxylase (pycA), adenylosuccinate lyase (purB), acylCoA dehydrogenase (acdA) and NADH dehydrogenase (ndh) allowed evaluation of metabolic changes in correlation to product formation and bioelectrochemical analysis. In comparison to control, poised circumstances showed marked influence on product profile with up-regulation of key enzymes involved in pyruvate metabolism. EF poised with - 0.8 V and -0.6 V enhanced bio-hydrogen production by 6 folds and 4 folds respectively. Concomitantly, -0.8 V resulted in maximum ethanol and acetic acid production whilst, -0.6 V and + 0.6 V resulted in maximum lactic acid and succinic acid production respectively. The transcripts for genes associated synthesis were upregulated in the respected poised reactors.