Han Zongchen, Hao Jie, Zou Dian, Sun Zhikang, Zhang Zekun, Niu Chenqi, Lu Qi, Huang Kuo, Ye Changwen, Wei Xuetuan
Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou, China.
Inner Mongolia Kunming Cigarettes LLC, Hohhot, China.
Front Microbiol. 2025 Jul 9;16:1603337. doi: 10.3389/fmicb.2025.1603337. eCollection 2025.
The combustion of excessive starch in tobacco leaves leads to more harmful substances, adversely affecting the sensory properties of tobacco and posing significant risks to human health. Therefore, there is an urgent need to develop specific amylases targeting tobacco starch to address these issues. In this study, 5 different -amylase genes were selected for recombinant expression in BAX-5, and the (derived from MK10163) was confirmed to be the optimal gene. Then, the -amylase activity was further increased by screening host bacteria BAX-5 and signal peptides SP (derived from the gene of 168). Subsequently, the -amylase properties were characterized, such as temperature tolerance, pH tolerance and metal ion. Through replacement of culture medium, the recombinant strain BAX-5/PT-17SP produced the maximum -amylase activity of 904.91 IU/mL, which was about 4 times higher than that of the original culture medium. Finally, the -amylase Amy (LC) was applied to the enzyme treatment of tobacco leaves, and the evaluation results showed that -amylase Amy (LC) could play a positive role in reducing damage and enhancing quality of cigarettes. This research provides a novel enzymatic resource for the development of amylases, and it has enormous market potential and application value.
烟叶中过量淀粉的燃烧会产生更多有害物质，对烟草的感官特性产生不利影响，并对人类健康构成重大风险。因此，迫切需要开发针对烟草淀粉的特异性淀粉酶来解决这些问题。在本研究中，选择了5个不同的α-淀粉酶基因在BAX-5中进行重组表达，其中（源自MK10163）被确认为最佳基因。然后，通过筛选宿主菌BAX-5和信号肽SP（源自168基因）进一步提高α-淀粉酶活性。随后，对α-淀粉酶的特性进行了表征，如耐热性、耐pH值和金属离子。通过更换培养基，重组菌株BAX-5/PT-17SP产生的最大α-淀粉酶活性为904.91 IU/mL，约为原始培养基的4倍。最后，将α-淀粉酶Amy（LC）应用于烟叶的酶处理，评价结果表明α-淀粉酶Amy（LC）在减少损伤和提高卷烟品质方面可发挥积极作用。本研究为淀粉酶的开发提供了一种新型酶资源，具有巨大的市场潜力和应用价值。
