Department of Urology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Med Sci. 2021 Sep;46(5):339-346. doi: 10.30476/ijms.2020.84462.1426.
Regulatory T cells (Tregs) play a key role in the progression of tumors. These cells express forkhead box P3 (FOXP3) and cytotoxic T-lymphocyte-associated protein 4 (CTLA4), which are the potential targets for cancer immunotherapy. The present study aimed to evaluate and transcripts in patients with bladder cancer (BC) compared with healthy individuals.
Transcripts of and genes in the peripheral blood mononuclear cells (PBMCs) of 50 patients with histologically confirmed BC and 50 healthy individuals were assessed at the Institute for Cancer Research, Shiraz University of Medical Sciences (Shiraz, Iran) during 2014-2016. RNA was extracted from PBMCs, then cDNA was synthesized and subjected to quantitative real-time PCR (qRT-PCR) using appropriate primers. Statistical analysis was performed using SPSS software (version 21.0).
Significantly higher amounts of and gene transcripts were found in the peripheral blood of BC patients compared with healthy individuals. The expression of both genes was significantly higher in patients with non-invasive and grade I/II BC. The median of and transcript expressions was 3.74 and 5.39, respectively, in non-invasive BC patients, which was significant compared with the control group (P=0.0016 and P=0.009, respectively). The median of target gene mRNA expression in grade I/II BC patients was 2.9 for and 6.61 for , which was significant compared with the controls (P=0.013 and P=0.0037, respectively).
This study highlights the functional activity of Tregs in early stages of bladder cancer and showed the importance of CTLA4 and FOXP3, when it comes to screening BC.
调节性 T 细胞（Tregs）在肿瘤的进展中起着关键作用。这些细胞表达叉头框 P3（FOXP3）和细胞毒性 T 淋巴细胞相关蛋白 4（CTLA4），它们是癌症免疫治疗的潜在靶点。本研究旨在评估与健康个体相比，膀胱癌（BC）患者外周血单个核细胞（PBMCs）中 和 转录本的表达情况。
2014 年至 2016 年期间，在伊朗 Shiraz 大学医学科学研究所（Shiraz），评估了 50 例经组织学证实的 BC 患者和 50 例健康个体的 PBMCs 中 和 基因的转录本。从 PBMCs 中提取 RNA，然后使用适当的引物合成 cDNA 并进行定量实时 PCR（qRT-PCR）。使用 SPSS 软件（版本 21.0）进行统计学分析。
与健康个体相比，BC 患者外周血中 和 基因转录本的含量明显更高。非浸润性和 I/II 级 BC 患者的两种基因表达均显著升高。非浸润性 BC 患者的 和 转录本表达中位数分别为 3.74 和 5.39，与对照组相比差异有统计学意义（P=0.0016 和 P=0.009）。I/II 级 BC 患者靶基因 mRNA 表达的中位数分别为 2.9 用于 和 6.61 用于 ，与对照组相比差异有统计学意义（P=0.013 和 P=0.0037）。
本研究强调了 Tregs 在膀胱癌早期阶段的功能活性，并表明 CTLA4 和 FOXP3 在膀胱癌筛查中具有重要意义。
