Gülen Dumrul, Şafak Birol, Erdal Berna, Günaydın Betül
Department of Medical Microbiology, Tekirdağ Namık Kemal University, Faculty of Medicine, Tekirdağ, Turkey.
Iran J Microbiol. 2021 Jun;13(3):345-351. doi: 10.18502/ijm.v13i3.6397.
The frequency of multiple resistant bacterial infections, including carbapenems, is increasing worldwide. As the decrease in treatment options causes difficulties in treatment, interest in new antimicrobials is increasing. One of the promising natural ingredients is curcumin. It is known to be effective in bacteria such as through efflux pump inhibition, toxin inhibition and enzymes. However, because its bioavailability is poor, it seffectiveness occurs in combination with antibiotics. In the study, the interaction of meropenem and curcumin in carbapenemase producing strains of was tested.
Thirty-nine isolates, resistant to meropenem, were used in this study. From those 15 MBL, 6 KPC, 17 OXA-48 and 1 AmpC resistance pattern were detected by combination disk method. Meropenem and Curcumin MIC values were determined by liquid microdilution. Checkerboard liquid microdilution was used to determine the synergy between meropenem and curcumin.
Synergistic effects were observed in 4 isolates producing MBL, 3 isolates producing KPC, 4 isolates producing OXA-48, and 1 isolates producing AmpC (totally 12 isolates) according to the calculated FICI. No antagonistic effects were observed in any isolates.
Curcumin was thought to be an alternative antimicrobial in combination therapies that would positively contribute to the treatment of bacterial infection. The effectiveness of this combination should be confirmed by other and clinical studies.
包括碳青霉烯类在内的多重耐药细菌感染在全球范围内的发生率正在上升。由于治疗选择的减少导致治疗困难，人们对新型抗菌药物的兴趣日益增加。姜黄素是一种有前景的天然成分之一。已知它通过抑制外排泵、毒素和酶等对细菌有效。然而，由于其生物利用度差，它与抗生素联合使用时才有效。在本研究中，测试了美罗培南与姜黄素在产碳青霉烯酶菌株中的相互作用。
本研究使用了39株对美罗培南耐药的分离株。通过联合纸片法从这些菌株中检测到15株金属β-内酰胺酶（MBL）、6株KPC、17株OXA-48和1株AmpC耐药模式。通过液体微量稀释法测定美罗培南和姜黄素的最低抑菌浓度（MIC）值。采用棋盘微量稀释法测定美罗培南与姜黄素之间的协同作用。
根据计算的FICI，在4株产MBL、3株产KPC、4株产OXA-48和1株产AmpC的分离株（共12株）中观察到协同效应。在任何分离株中均未观察到拮抗作用。
姜黄素被认为是联合治疗中的一种替代抗菌药物，对细菌感染的治疗将有积极贡献。这种联合用药的有效性应通过其他研究和临床研究来证实。
