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RSV 通过 NODAL 信号通路促进上皮神经内分泌表型分化。

RSV Promotes Epithelial Neuroendocrine Phenotype Differentiation through NODAL Signaling Pathway.

机构信息

Department of Medical Microbiology, School of Basic Medical Sciences, Central South University, Changsha, 410078 Hunan, China.

China-Africa Research Center of Infectious Diseases, School of Basic Medical Sciences, Central South University, Changsha, 410078 Hunan, China.

出版信息

Biomed Res Int. 2021 Sep 8;2021:9956078. doi: 10.1155/2021/9956078. eCollection 2021.

DOI:10.1155/2021/9956078
PMID:34541002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8445725/
Abstract

BACKGROUND

Respiratory syncytial virus (RSV) infects infants and children, predisposing them to development of asthma during adulthood. Epithelial neuroendocrine phenotypes may be associated with development of asthma. This study hopes to ascertain if RSV infection promotes epithelial neuroendocrine phenotypes through the NODAL signaling pathway.

METHODS

The GSE6802 data set was obtained from the GEO database, and the differential genes were analyzed using the language. An in vitro model was constructed with RSV infected human respiratory epithelial cells, and then real-time qPCR and immunofluorescence were used to detect the expression of different epithelial biomarkers and airway neuropeptides. The acute and chronic infection model of RSV infection was established by intranasal injection of RSV into guinea pigs. Immunohistochemistry and Western blot were used to detect the expression of pulmonary neuroendocrine cells markers ENO2 and neuropeptides.

RESULTS

The expression levels of ENO2, SP, CGRP, and NODAL/ACTRII were significantly higher in the RSV infection group than those of the control group, which were abrogated by siRNA-NODAL. In vivo, we found that the expression levels of ENO2, SP, and CGRP were significantly higher than that of the control group.

CONCLUSION

RSV promotes epithelial neuroendocrine phenotypes through the NODAL signaling pathway.

摘要

背景

呼吸道合胞病毒（RSV）感染婴儿和儿童，使他们在成年后易患哮喘。上皮神经内分泌表型可能与哮喘的发展有关。本研究希望通过 NODAL 信号通路确定 RSV 感染是否通过 NODAL 信号通路促进上皮神经内分泌表型。

方法

从 GEO 数据库中获取 GSE6802 数据集，并使用 语言分析差异基因。用 RSV 感染人呼吸道上皮细胞构建体外模型，然后用实时 qPCR 和免疫荧光检测不同上皮标志物和气道神经肽的表达。通过 RSV 滴鼻感染建立 RSV 感染的急性和慢性感染模型。用免疫组化和 Western blot 检测肺神经内分泌细胞标志物 ENO2 和神经肽的表达。

结果

RSV 感染组 ENO2、SP、CGRP 和 NODAL/ACTRII 的表达水平明显高于对照组，而 siRNA-NODAL 则使其降低。在体内，我们发现 ENO2、SP 和 CGRP 的表达水平明显高于对照组。

结论

RSV 通过 NODAL 信号通路促进上皮神经内分泌表型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/af833fe5469a/BMRI2021-9956078.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/13bf272c01ae/BMRI2021-9956078.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/5d2a364e79b9/BMRI2021-9956078.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/382e11853f8e/BMRI2021-9956078.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/8643f5c5815b/BMRI2021-9956078.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/af833fe5469a/BMRI2021-9956078.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/13bf272c01ae/BMRI2021-9956078.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/5d2a364e79b9/BMRI2021-9956078.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/382e11853f8e/BMRI2021-9956078.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/8643f5c5815b/BMRI2021-9956078.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b0/8445725/af833fe5469a/BMRI2021-9956078.005.jpg

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