Wu Xiuxiu, Qi Huijuan, Yang Yan, Yin Yunhong, Ma Dedong, Li Hao, Qu Yiqing
Department of Respiratory Medicine, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
Intensive Care Unit, The People's Hospital of Yucheng, Yucheng, Shandong 251200, P.R. China.
Mol Med Rep. 2016 Jan;13(1):167-73. doi: 10.3892/mmr.2015.4518. Epub 2015 Nov 6.
The present study was designed to examine the expression and function of matrix metalloproteinase‑19 (MMP‑19), which is downregulated following respiratory syncytial virus (RSV) infection. The diverse expression levels of MMP were examined using a designed cDNA expression array. The expression and secretion of MMP‑19 was examined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and ELISA, respectively. The proliferation of epithelial cells and lung fibroblasts were examined using flow cytometry. The epithelial‑mesenchymal transition (EMT) was also examined by performing western blot and RT‑qPCR analyses. The results of the cDNA assay showed that infection with RSV resulted in the abnormal expression of certain metalloproteinases. Among these, the expression of MMP‑19 decreased 3 and 7 days following infection. By using flow cytometric, western blot and RT‑qPCR analyses, the present study demonstrated that the downregulation of MMP‑19 inhibited the proliferation of epithelial cells, promoted the EMT and induced the proliferation of lung fibroblasts. Taken together, the findings of the present study suggested that the downregulation of MMP‑19 following RSV infection may be associated with the development of airway hyper‑responsiveness.
本研究旨在检测基质金属蛋白酶-19（MMP-19）的表达及功能，其在呼吸道合胞病毒（RSV）感染后表达下调。使用设计好的cDNA表达阵列检测MMP的不同表达水平。分别采用逆转录-定量聚合酶链反应（RT-qPCR）分析和酶联免疫吸附测定（ELISA）检测MMP-19的表达和分泌。使用流式细胞术检测上皮细胞和肺成纤维细胞的增殖。还通过蛋白质免疫印迹法和RT-qPCR分析检测上皮-间质转化（EMT）。cDNA检测结果显示，RSV感染导致某些金属蛋白酶表达异常。其中，感染后3天和7天MMP-19的表达下降。通过流式细胞术、蛋白质免疫印迹法和RT-qPCR分析，本研究表明MMP-19的下调抑制上皮细胞增殖，促进EMT并诱导肺成纤维细胞增殖。综上所述，本研究结果提示RSV感染后MMP-19的下调可能与气道高反应性的发展有关。
