从质体中分离和检测叶绿素分解代谢物羟化活性
Isolation and Detection of the Chlorophyll Catabolite Hydroxylating Activity from Chromoplasts.
作者信息
Hauenstein Mareike, Hörtensteiner Stefan
机构信息
Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
出版信息
Bio Protoc. 2017 Sep 20;7(18):e2561. doi: 10.21769/BioProtoc.2561.
Abstract
Hydroxylation of chlorophyll catabolites at the so-called C3 position ( Hauenstein , 2016 ) is commonly found in all plant species analyzed to date. Here we describe an hydroxylation assay using chromoplast membranes as a source of the hydroxylating activity, which converts the substrate FCC ( Fluorescent Chlorophyll Catabolite) ( Mühlecker , 2000 ) to FCC-OH.
摘要
在迄今分析的所有植物物种中，叶绿素分解代谢产物在所谓的C3位置的羟基化作用（豪恩施泰因，2016年）普遍存在。在此，我们描述了一种羟基化测定方法，该方法使用有色体膜作为羟基化活性的来源，可将底物FCC（荧光叶绿素分解代谢产物）（米尔克，2000年）转化为FCC-OH。
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本文引用的文献
1
A Role for TIC55 as a Hydroxylase of Phyllobilins, the Products of Chlorophyll Breakdown during Plant Senescence.TIC55作为叶卟啉羟化酶的作用，叶卟啉是植物衰老过程中叶绿素分解的产物。
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2
A liquid chromatography-mass spectrometry platform for the analysis of phyllobilins, the major degradation products of chlorophyll in Arabidopsis thaliana.一种用于分析拟南芥中叶绿素主要降解产物叶胆素的液相色谱-质谱平台。
Plant J. 2016 Nov;88(3):505-518. doi: 10.1111/tpj.13253. Epub 2016 Sep 8.
3
Breakdown of Chlorophyll in Higher Plants--Phyllobilins as Abundant, Yet Hardly Visible Signs of Ripening, Senescence, and Cell Death.高等植物中叶绿素的分解——叶胆色素作为成熟、衰老和细胞死亡的丰富但难以察觉的标志
Angew Chem Int Ed Engl. 2016 Apr 11;55(16):4882-907. doi: 10.1002/anie.201508928. Epub 2016 Feb 26.
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