Hegenauer Volker, Welz Max, Körner Max, Albert Markus
Center for Plant Molecular Biology, University of Tübingen, Tübingen, Germany.
Bio Protoc. 2017 Apr 20;7(8):e2243. doi: 10.21769/BioProtoc.2243.
spp. are widespread obligate holoparasitic plants with a broad host spectrum. Rootless penetrates host stems with so called haustoria to form a direct connection to the host vascular tissue ( Dawson , 1994 ; Lanini and Kogan, 2005; Kaiser , 2015 ). This connection allows a steady uptake of water, assimilates and essential nutrients from the host plant and therefore enables growth and proliferation. To quantify the parasites' ability to grow on potential host plants one can use the quantitative growth assay ( Hegenauer , 2016 ) described herein, which exclusively utilizes fresh weight measurement as readout.
该属植物是广泛分布的专性全寄生植物,寄主范围广泛。无根属植物通过所谓的吸器穿透寄主茎干,与寄主维管组织形成直接连接(道森,1994年;拉尼尼和科根,2005年;凯泽,2015年)。这种连接使得能够从寄主植物中稳定摄取水分、同化产物和必需营养物质,从而实现生长和繁殖。为了量化寄生虫在潜在寄主植物上生长的能力,可以使用本文所述的定量生长测定法(黑格纳,2016年),该方法仅以鲜重测量作为读数。