• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Immunoprecipitation of Cell Surface Proteins from Gram-negative Bacteria.革兰氏阴性菌细胞表面蛋白的免疫沉淀
Bio Protoc. 2017 May 5;7(9):e2250. doi: 10.21769/BioProtoc.2250.
2
Identification of the immunoproteome of the meningococcus by cell surface immunoprecipitation and MS.通过细胞表面免疫沉淀和 MS 鉴定脑膜炎球菌的免疫蛋白质组。
Microbiology (Reading). 2014 Feb;160(Pt 2):429-438. doi: 10.1099/mic.0.071829-0. Epub 2013 Nov 25.
3
Genetic Similarity of Gonococcal Homologs to Meningococcal Outer Membrane Proteins of Serogroup B Vaccine.淋病奈瑟菌同源物与 B 群脑膜炎奈瑟球菌外膜蛋白的遗传相似性。
mBio. 2019 Sep 10;10(5):e01668-19. doi: 10.1128/mBio.01668-19.
4
Characterization of the key antigenic components and pre-clinical immune responses to a meningococcal disease vaccine based on Neisseria lactamica outer membrane vesicles.基于乳酸奈瑟菌外膜囊泡的脑膜炎球菌病疫苗关键抗原成分的表征及临床前免疫反应
Hum Vaccin. 2008 Jan-Feb;4(1):23-30. doi: 10.4161/hv.4.1.4806. Epub 2007 Jul 29.
5
Approach to the Discovery, Development, and Evaluation of a Novel Neisseria meningitidis Serogroup B Vaccine.新型B群脑膜炎奈瑟菌疫苗的发现、研发与评估方法
Methods Mol Biol. 2016;1403:445-69. doi: 10.1007/978-1-4939-3387-7_25.
6
Genetic shifts of Neisseria meningitidis serogroup B antigens and the quest for a broadly cross-protective vaccine.B 群脑膜炎奈瑟氏球菌抗原的遗传转变与广泛交叉保护疫苗的探索。
Expert Rev Vaccines. 2010 Oct;9(10):1203-17. doi: 10.1586/erv.10.116.
7
Role of factor H binding protein in Neisseria meningitidis virulence and its potential as a vaccine candidate to broadly protect against meningococcal disease.因子 H 结合蛋白在脑膜炎奈瑟菌毒力中的作用及其作为广泛预防脑膜炎球菌病疫苗候选物的潜力。
Microbiol Mol Biol Rev. 2013 Jun;77(2):234-52. doi: 10.1128/MMBR.00056-12.
8
Effectiveness of a Serogroup B and C Meningococcal Vaccine Developed in Cuba.古巴研发的 B 群和 C 群脑膜炎球菌疫苗的有效性。
MEDICC Rev. 2018 Jul;20(3):22-29. doi: 10.37757/MR2018.V20.N3.6.
9
Strategies for development of universal vaccines against meningococcal serogroup B disease: the most promising options and the challenges evaluating them.针对B群脑膜炎球菌疾病开发通用疫苗的策略：最具前景的选择及其评估挑战。
Hum Vaccin. 2007 Nov-Dec;3(6):290-4. doi: 10.4161/hv.4513. Epub 2007 May 30.
10
The Development of a Vaccine Against Meningococcus B Using Reverse Vaccinology.利用反向疫苗学开发 B 型脑膜炎球菌疫苗。
Front Immunol. 2019 Apr 16;10:751. doi: 10.3389/fimmu.2019.00751. eCollection 2019.

引用本文的文献

1
Enhancement of clinical signs in C3H/HeJ mice vaccinated with a highly immunogenic Leptospira methyl-accepting chemotaxis protein following challenge.经高度免疫原性钩端螺旋体甲基接受趋化蛋白免疫接种的 C3H/HeJ 小鼠在攻毒后临床体征增强。
PLoS Negl Trop Dis. 2024 Sep 23;18(9):e0012155. doi: 10.1371/journal.pntd.0012155. eCollection 2024 Sep.
2
Challenges for the development of a universal vaccine against leptospirosis revealed by the evaluation of 22 vaccine candidates.通过对 22 种候选疫苗的评估，揭示了开发通用钩端螺旋体病疫苗所面临的挑战。
Front Cell Infect Microbiol. 2022 Oct 7;12:940966. doi: 10.3389/fcimb.2022.940966. eCollection 2022.

本文引用的文献

1
Identification of the immunoproteome of the meningococcus by cell surface immunoprecipitation and MS.通过细胞表面免疫沉淀和 MS 鉴定脑膜炎球菌的免疫蛋白质组。
Microbiology (Reading). 2014 Feb;160(Pt 2):429-438. doi: 10.1099/mic.0.071829-0. Epub 2013 Nov 25.
2
Predicted strain coverage of a meningococcal multicomponent vaccine (4CMenB) in Europe: a qualitative and quantitative assessment.预测脑膜炎球菌多组份疫苗（4CMenB）在欧洲的菌株覆盖率：定性和定量评估。
Lancet Infect Dis. 2013 May;13(5):416-25. doi: 10.1016/S1473-3099(13)70006-9. Epub 2013 Feb 13.
3
SignalP 4.0: discriminating signal peptides from transmembrane regions.信号肽预测工具SignalP 4.0：区分信号肽与跨膜区域。
Nat Methods. 2011 Sep 29;8(10):785-6. doi: 10.1038/nmeth.1701.
4
Exploring the proteome of meningococcal outer membrane vesicle vaccines.探索脑膜炎奈瑟菌外膜囊泡疫苗的蛋白质组。
Proteomics Clin Appl. 2007 Sep;1(9):1198-210. doi: 10.1002/prca.200700204. Epub 2007 Aug 17.
5
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes.PSORTb 3.0：通过改进定位亚类和提高对所有原核生物的预测能力，改善了蛋白质亚细胞定位预测。
Bioinformatics. 2010 Jul 1;26(13):1608-15. doi: 10.1093/bioinformatics/btq249. Epub 2010 May 13.
6
Gneg-mPLoc: a top-down strategy to enhance the quality of predicting subcellular localization of Gram-negative bacterial proteins.Gneg-mPLoc：一种提升革兰氏阴性细菌蛋白亚细胞定位预测质量的自顶向下策略。
J Theor Biol. 2010 May 21;264(2):326-33. doi: 10.1016/j.jtbi.2010.01.018. Epub 2010 Jan 20.
7
Towards the immunoproteome of Neisseria meningitidis.迈向脑膜炎奈瑟菌免疫蛋白质组学。
PLoS One. 2009 Jun 16;4(6):e5940. doi: 10.1371/journal.pone.0005940.
8
Signal-CF: a subsite-coupled and window-fusing approach for predicting signal peptides.信号-CF：一种用于预测信号肽的亚位点耦合和窗口融合方法。
Biochem Biophys Res Commun. 2007 Jun 8;357(3):633-40. doi: 10.1016/j.bbrc.2007.03.162. Epub 2007 Apr 5.
9
Prediction of protein subcellular localization.蛋白质亚细胞定位预测
Proteins. 2006 Aug 15;64(3):643-51. doi: 10.1002/prot.21018.
10
PrediSi: prediction of signal peptides and their cleavage positions.PrediSi：信号肽及其切割位点的预测
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W375-9. doi: 10.1093/nar/gkh378.

革兰氏阴性菌细胞表面蛋白的免疫沉淀

Immunoprecipitation of Cell Surface Proteins from Gram-negative Bacteria.

作者信息

Cunha Carlos Eduardo Pouey, Newcombe Jane, Dellagostin Odir Antonio, McFadden Johnjoe

机构信息

School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey,Guildford, Surrey GU2 7XH, UK.

Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brasil.

出版信息

Bio Protoc. 2017 May 5;7(9):e2250. doi: 10.21769/BioProtoc.2250.

DOI:10.21769/BioProtoc.2250
PMID:34541240
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8410285/
Abstract

The meningococcus () remains an important threat to human healthworldwide. This Gram-negative bacterium causes elevated disabilities and mortality in infectedindividuals. Despite several available vaccines, currently there is no universal vaccine against allcirculating meningococcal strains ( Vogel , 2013 ). Herein, we describe a new protocol that iscapable of identifying only cell surface exposed proteins that play a role in immunity, providing thisresearch field with a more straightforward approach to identify novel vaccine targets. Even though is used as a model in the protocol herein described, this protocol can be used for anyGram-negative bacteria provided modifications and optimizations are carried out to adapt it to differentbacterial and disease characteristics (, membrane fragility, growth methods, serum antibody levels,).

摘要

脑膜炎双球菌（）仍然是全球人类健康的一个重要威胁。这种革兰氏阴性细菌会导致受感染个体的残疾率和死亡率上升。尽管有几种可用的疫苗，但目前尚无针对所有流行的脑膜炎球菌菌株的通用疫苗（Vogel，2013年）。在此，我们描述了一种新方案，该方案能够仅识别在免疫中起作用的细胞表面暴露蛋白，为该研究领域提供了一种更直接的方法来识别新型疫苗靶点。尽管本文所述方案中使用作为模型，但只要进行修改和优化以使其适应不同的细菌和疾病特征（如膜脆性、生长方法、血清抗体水平等），该方案可用于任何革兰氏阴性细菌。