Sattgast Lara H, Branscum Adam J, Jimenez Vanessa A, Newman Natali, Grant Kathleen A, Turner Russell T, Iwaniec Urszula T
Skeletal Biology Laboratory, School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA.
Biostatistics Program, School of Biological and Population Health Sciences, Oregon State University, Corvallis, OR, USA.
Bone Rep. 2021 Sep 5;15:101126. doi: 10.1016/j.bonr.2021.101126. eCollection 2021 Dec.
Development of optimal bone mass during early adulthood is determined by the balance between bone formation and resorption. The utility of minimally invasive biomarkers for monitoring bone turnover balance in maturing non-human primates has received limited attention. This study evaluated the biological variation of osteocalcin (a marker of bone formation), carboxyterminal cross-linking telopeptide of type 1 collagen (CTX, a marker of bone resorption), and the ratio of osteocalcin to CTX (reflecting bone turnover balance), in 136 rhesus and cynomolgus macaques aged 3.8-11.6 years. In a subsample of the animals (n = 28), blood samples were collected at monthly intervals over 4 months. Between-subject analysis revealed that there were no sex or species differences for CTX. Osteocalcin and the ratio of osteocalcin to CTX were higher in males than in females, and in rhesus macaques than in cynomolgus macaques. There were no changes in osteocalcin, CTX, or the ratio of osteocalcin to CTX across 4 months for any of the groups. In contrast, there was considerable within-subject variation in osteocalcin and CTX concentrations. However, differences in values exhibited no discernible pattern, suggesting that within-subject variation can be reduced by averaging repeat measurements. In summary, the data provide reference values for male and female rhesus and cynomolgus macaques and support the utility of osteocalcin and CTX as biomarkers to monitor bone turnover at the population level.
成年早期最佳骨量的发育取决于骨形成与骨吸收之间的平衡。在成熟的非人灵长类动物中，用于监测骨转换平衡的微创生物标志物的效用受到的关注有限。本研究评估了136只年龄在3.8至11.6岁之间的恒河猴和食蟹猴中骨钙素（一种骨形成标志物）、1型胶原羧基末端交联肽（CTX，一种骨吸收标志物）以及骨钙素与CTX的比值（反映骨转换平衡）的生物学变异。在动物的一个子样本（n = 28）中，在4个月内每月采集一次血样。受试者间分析显示，CTX不存在性别或物种差异。雄性的骨钙素以及骨钙素与CTX的比值高于雌性，恒河猴的高于食蟹猴。任何一组在4个月内骨钙素、CTX或骨钙素与CTX的比值均无变化。相比之下，骨钙素和CTX浓度在受试者内存在相当大的变异。然而，数值差异没有明显的模式，这表明通过对重复测量值求平均值可以减少受试者内变异。总之，这些数据为雄性和雌性恒河猴和食蟹猴提供了参考值，并支持骨钙素和CTX作为在群体水平监测骨转换的生物标志物的效用。
