Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA, USA.
J Feline Med Surg. 2022 Jun;24(6):580-586. doi: 10.1177/1098612X211044986. Epub 2021 Sep 20.
The aim of this study was to investigate the spatial distribution of neutrophil extracellular traps (NETs) in cardiogenic arterial thromboembolism (CATE). Specifically, we aimed to examine the related structural features of NETs in feline arterial thrombi in relation to their arterial locations.
Paraffin-embedded aortic bifurcations from nine cats with hypertrophic cardiomyopathy (four with CATE and five without) were deparaffinized, and NETs were identified by immunodetection based on colocalization of cell-free DNA, citrullinated histone H3 and neutrophil elastase. The distribution of NETs in thrombi within the aortic bifurcations and common iliac arteries (CIAs) was compared based on their proximity to the descending aorta (proximal, mid, distal). Ten random fields per section were captured at × 10 and × 20 magnification for each section of the clot and analyzed.
The distributions of NETs in thrombi within the aortic bifurcation and CIAs were found to differ in relation to their assigned zones (proximal, mid, distal; P = 0.04); NETs were concentrated mostly in the proximal region in the aortic bifurcations (47.56%, interquartile range [IQR] 14.07-77.95) and CIAs (44.69%, IQR 24.65-85.28), compared with the distal regions (2.69%, IQR 0.10-50.04 [P = 0.027]; 7.08%, IQR 1.27-59.33 [P = 0.02]).
The variation in NET distribution within arterial thrombi may shed light on the pathogenesis of thrombus growth. This may be due to possible neutrophil entrapment or variations in shear stress.
本研究旨在探讨中性粒细胞胞外诱捕网（NETs）在心源性动脉血栓栓塞（CATE）中的空间分布。具体而言，我们旨在研究猫动脉血栓中与动脉位置相关的 NETs 的相关结构特征。
对 9 只患有肥厚型心肌病的猫的主动脉分叉处的石蜡包埋组织进行脱蜡，通过无细胞 DNA、瓜氨酸化组蛋白 H3 和中性粒细胞弹性蛋白酶的共定位，基于免疫检测识别 NETs。根据其与降主动脉（近侧、中侧、远侧）的接近程度，比较主动脉分叉处和髂总动脉（CIA）内血栓中 NETs 的分布。对每个血栓切片的 10 个随机视野在 ×10 和 ×20 放大倍数下进行捕获，并进行分析。
发现主动脉分叉处和 CIA 内血栓中 NETs 的分布与它们的指定区域（近侧、中侧、远侧）不同（P=0.04）；NETs 主要集中在主动脉分叉处（47.56%，四分位距 [IQR] 14.07-77.95）和 CIA（44.69%，IQR 24.65-85.28）的近侧区域，而远侧区域较少（2.69%，IQR 0.10-50.04 [P=0.027]；7.08%，IQR 1.27-59.33 [P=0.02]）。
动脉血栓内 NET 分布的变化可能揭示血栓生长的发病机制。这可能是由于可能的中性粒细胞捕获或剪切应力的变化。
