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患有肥厚型心肌病和心原性动脉血栓栓塞症的猫的循环中性粒细胞细胞外陷阱。

Circulating neutrophil extracellular traps in cats with hypertrophic cardiomyopathy and cardiogenic arterial thromboembolism.

机构信息

Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.

Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, Davis, California, USA.

出版信息

J Vet Intern Med. 2023 Mar;37(2):490-502. doi: 10.1111/jvim.16676. Epub 2023 Mar 23.

DOI:10.1111/jvim.16676

PMID:36951591

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10061180/

Abstract

BACKGROUND

Cats with hypertrophic cardiomyopathy (HCM) are at risk of cardiogenic arterial thromboembolism (CATE). Neutrophil extracellular traps (NETs) may be a potential biomarker and therapeutic target for cardiomyopathy in cats.

HYPOTHESIS/OBJECTIVES: Characterize NETs in cats with HCM or CATE. We hypothesized that circulating NETs assessed in the form of cell-free DNA (cfDNA) and citrullinated histone H3 (citH3) are increased in cats with HCM and CATE and associated with reported predisposing factors for thrombus formation.

ANIMALS

Eighty-five cats including client-owned cats with HCM and CATE and staff- and student-owned clinically healthy cats without HCM.

METHODS

After echocardiographic evaluations, NETs were measured as cfDNA and citH3.

RESULTS

Cats with CATE had significant increases in cfDNA (11.2 ng/μL; interquartile range [IQR], 8.1 to 29.6) compared to those without HCM (8.2 ng/μL; IQR, 5.7 to 11.7 μL; P = .01) and were responsible for 75% to 83% of cases with cfDNA fragments sized 100 to 2000 base pairs. Citrullinated histone 3, detected in 52% of cats with HCM (31.1 ng/mL; IQR, 16.9 to 29.8), was significantly lower than in those with CATE (48.2 ng/mL; IQR, 34.2 to 60.2; P = .007). The citH3 concentrations correlated significantly with reported risk factors of CATE, such as left atrial auricular velocity.

CONCLUSIONS AND CLINICAL IMPORTANCE

Neutrophil extracellualr traps, especially citH3, are increased in cats with HCM and CATE. They may serve as a novel therapeutic target and biomarker of thrombosis in cats with HCM.

摘要

背景

患有肥厚型心肌病（HCM）的猫有发生心源性动脉血栓栓塞（CATE）的风险。中性粒细胞胞外诱捕网（NETs）可能是猫心肌病的一个潜在生物标志物和治疗靶点。

假设/目的：描述患有 HCM 或 CATE 的猫的 NETs。我们假设以游离 DNA（cfDNA）和瓜氨酸化组蛋白 H3（citH3）的形式评估循环 NETs 在患有 HCM 和 CATE 的猫中增加，并与报告的血栓形成易患因素相关。

动物

包括患有 HCM 和 CATE 的患宠猫、无 HCM 的员工和学生所有的临床健康猫在内的 85 只猫。

方法

在超声心动图评估后，测量 NETs 作为 cfDNA 和 citH3。

结果

患有 CATE 的猫的 cfDNA 显著增加（11.2ng/μL；四分位间距 [IQR]，8.1 至 29.6），与无 HCM 的猫相比（8.2ng/μL；IQR，5.7 至 11.7ng/μL；P=.01），并导致 75%至 83%的 cfDNA 片段大小为 100 至 2000 个碱基对的病例。在 52%患有 HCM 的猫中检测到瓜氨酸化组蛋白 3（31.1ng/mL；IQR，16.9 至 29.8），显著低于患有 CATE 的猫（48.2ng/mL；IQR，34.2 至 60.2；P=.007）。citH3 浓度与 CATE 的报告风险因素（如左心房耳速度）显著相关。