Department of Chemistry, Faculty of Sciences, University of Ngaoundere, Ngaoundere, Cameroon.
Department of Chemical Engineering, School of Chemical Engineering and Mineral Industries, University of Ngaoundere, Ngaoundere, Cameroon.
Nat Prod Res. 2022 Aug;36(16):4132-4139. doi: 10.1080/14786419.2021.1976176. Epub 2021 Sep 20.
A new abietane-type diterpenoid, rubesanolidic acid (), alongside six known compounds including -sitosterol (), lupeol (), betulinic acid () ursolic acid (), -sitosterol 3--glucopyranoside () and stigmasterol 3-glucopyranoside () were isolated from the roots of through column chromatography. Their structures were elucidated from spectroscopic analyses (UV, IR, MS, 1D and 2D NMR) data and by comparison with data from previous studies. The extract and compounds were tested for their -amylase inhibition. The extract was more active than the isolated compounds with a percentage inhibition of 51.0 ± 2.5% at 400 µg/mL and was the only sample showing above 50% inhibition at this dose. Amongst the isolated compounds and at the dose of 400 µg/mL, the new diterpenoid Rubesanolidic acid exibited the highest percentage inhibition of -amylase of 38.2 ± 2.0% while -sitosterol showed the lowest inhibition of 9.6 ± 0.5%. The results indicate that is a potential source of antidiabetic compounds.
从 根部分离得到一个新的贝壳杉烷型二萜，鲁比沙诺酸（），以及 6 个已知化合物，包括β-谷甾醇（），羽扇豆醇（），白桦脂酸（），熊果酸（），β-谷甾醇 3-O-β-D-吡喃葡萄糖苷（）和豆甾醇 3-O-β-D-吡喃葡萄糖苷（）。通过光谱分析（UV、IR、MS、1D 和 2D NMR）数据和与以往研究数据的比较，确定了它们的结构。对提取物和化合物进行了 -淀粉酶抑制试验。提取物的活性高于分离得到的化合物，在 400μg/mL 时抑制率为 51.0±2.5%，是唯一在该剂量下抑制率超过 50%的样品。在所测试的化合物中，在 400μg/mL 的剂量下，新的二萜 Rubesanolidic acid 对 -淀粉酶的抑制率最高，为 38.2±2.0%，而β-谷甾醇的抑制率最低，为 9.6±0.5%。结果表明，是一种有潜力的抗糖尿病化合物的来源。
