Department of Chemistry, University of Mumbai, Vidyanagari, Mumbai, India.
Department of Life Sciences, University of Mumbai, Vidyanagari, Mumbai, India.
J Biomol Struct Dyn. 2022;40(23):12895-12907. doi: 10.1080/07391102.2021.1977701. Epub 2021 Sep 20.
Here, we report the physicochemical interaction among iron oxide nanoparticles (MNPs) and essential biomolecules, namely, serum albumin (BSA, HSA), collagen and deoxyribonucleic acid (DNA) in the presence of various cationic, anionic and non-ionic surfactants. Iron oxide nanoparticles are synthesized by the wet chemical process and are characterized by X-ray powder diffraction analysis (XRD), Fourier transform infrared spectroscopic, UV-Vis spectroscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy mapping studies . The conjugation of MNPs protein was analyzed using UV-Vis spectroscopy, fluorescence spectroscopy, circular dichroism technique and gel electrophoresis. The spectroscopic investigation illustrates the surfactant-dependent binding between MNPs and protein. Gel electrophoresis in the absence and presence of MNPs-surfactant systems has been used to study the impact on DNA structure. It was found that Tween 80 imparts better stability as well as biocompatibility to the synthesized MNPs. The findings offer extensive information on the influence of various surfactant coatings on MNP surfaces and their influence on vital biomolecules, making it useful for designing MNPs for biological applications.Communicated by Ramaswamy H. Sarma.
在这里，我们报告了氧化铁纳米粒子（MNPs）与基本生物分子（如血清白蛋白（BSA、HSA）、胶原蛋白和脱氧核糖核酸（DNA））之间在各种阳离子、阴离子和非离子表面活性剂存在下的物理化学相互作用。通过湿化学法合成氧化铁纳米粒子，并通过 X 射线粉末衍射分析（XRD）、傅里叶变换红外光谱、紫外-可见光谱、扫描电子显微镜和能量色散 X 射线光谱映射研究进行了表征。使用紫外-可见光谱、荧光光谱、圆二色性技术和凝胶电泳分析了 MNPs 与蛋白质的缀合。在有无 MNPs-表面活性剂系统的凝胶电泳中研究了其对 DNA 结构的影响。结果表明，吐温 80 赋予合成的 MNPs 更好的稳定性和生物相容性。这些发现提供了关于各种表面活性剂涂层对 MNP 表面的影响及其对重要生物分子的影响的广泛信息，这对于设计用于生物应用的 MNPs 非常有用。由 Ramaswamy H. Sarma 传达。
