BMSCs 关节腔内注射 miR-29a-3p 促进软骨自我修复并缓解大鼠骨关节炎疼痛。
Intra-Articular Injection of miR-29a-3p of BMSCs Promotes Cartilage Self-Repairing and Alleviates Pain in the Rat Osteoarthritis.
机构信息
Department of Orthopedics, Kunshan Affiliated Hospital of Nanjing University of Chinese Medicine, Kunshan, 215300, China.
Department of Orthopedics, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, 215300, China.
出版信息
Tissue Eng Regen Med. 2021 Dec;18(6):1045-1055. doi: 10.1007/s13770-021-00384-7. Epub 2021 Sep 20.
BACKGROUND
Stem cells intra-articular injection stagey indicated a potential therapeutic effect on improving the pathological progress of osteoarthritis (OA). However, the long-term effect of stem cells intra-articular injection on the cartilage regeneration remains unclear. miR-29a-3p is predicted to be a critical target for inhibiting insulin-like growth factor-1 expression and may aggravate the progression of OA.
METHODS
In this study, we investigated the therapeutic efficacy of intra-articular injection of bone marrow mesenchymal stem cells (BMSCs) transfected with miR-29a-3p inhibitor in OA.
RESULTS
miR-29a-3p inhibitor transfection did not influence cell viability of BMSCs, while the chondrogenic differentiation potential of BMSCs was significantly improved. Interestingly, intra-articular injection of BMSCs with miR-29a-3p inhibition significantly prevented articular cartilage degeneration by up-regulating the expression of Sox 9, Col-2a1, aggrecan and down-regulating the expression of matrix metalloproteinase, as well as relieved pain in OA.
CONCLUSION
The double effects on cartilage protection and pain relief indicated a great potential of intra-articular injection of miR-29a-3p inhibitor-transfected BMSCs for the treatment of OA.
背景
关节内注射干细胞策略表明对改善骨关节炎（OA）的病理进程具有潜在的治疗效果。然而，关节内注射干细胞对软骨再生的长期效果尚不清楚。miR-29a-3p 被预测为抑制胰岛素样生长因子-1 表达的关键靶点，可能加重 OA 的进展。
方法
本研究旨在探讨转染 miR-29a-3p 抑制剂的骨髓间充质干细胞（BMSCs）关节内注射治疗 OA 的疗效。
结果
miR-29a-3p 抑制剂转染并不影响 BMSCs 的细胞活力，而 BMSCs 的成软骨分化潜能显著提高。有趣的是，miR-29a-3p 抑制转染的 BMSCs 关节内注射通过上调 Sox9、Col-2a1、聚集蛋白聚糖和下调基质金属蛋白酶的表达显著防止了关节软骨退化，并缓解了 OA 的疼痛。
结论
对软骨保护和缓解疼痛的双重作用表明，关节内注射 miR-29a-3p 抑制剂转染的 BMSCs 治疗 OA 具有很大的潜力。
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