利用活细胞和固定的果蝇组织培养细胞对细胞骨架进行成像。
Imaging of the Cytoskeleton Using Live and Fixed Drosophila Tissue Culture Cells.
机构信息
Department of Biology, Reed College, Portland, OR, USA.
Department of Biology, University of Richmond, Richmond, VA, USA.
出版信息
Methods Mol Biol. 2022;2364:159-173. doi: 10.1007/978-1-0716-1661-1_8.
In recent years, the convergence of multiple technologies and experimental approaches has led to the expanded use of cultured Drosophila cells as a model system. Their ease of culture and maintenance, susceptibility to RNA interference, and imaging characteristics have led to extensive use in both traditional experimental approaches and high-throughput RNAi screens. Here we describe Drosophila S2 cell culture and preparation for live-cell and fixed-cell fluorescence microscopy and scanning electron microscopy.
近年来，多种技术和实验方法的融合促使培养的果蝇细胞被更广泛地用作模型系统。它们易于培养和维持，对 RNA 干扰敏感，以及成像特性，使得它们在传统的实验方法和高通量 RNAi 筛选中得到了广泛应用。在这里，我们描述了果蝇 S2 细胞的培养以及用于活细胞和固定细胞荧光显微镜和扫描电子显微镜的准备。
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