姜黄素改善老年大鼠心脏的血管生成：涉及 TSP1/NF-κB/VEGF-A 信号通路。
Curcumin improves angiogenesis in the heart of aged rats: Involvement of TSP1/NF-κB/VEGF-A signaling.
机构信息
Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Neurophysiology Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran; Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
出版信息
Microvasc Res. 2022 Jan;139:104258. doi: 10.1016/j.mvr.2021.104258. Epub 2021 Sep 17.
BACKGROUND
Cardiac aging is an irreversible process that is determined by a number of slowly deleterious changes in morphological and physiological properties of the heart. We investigated the effects of curcumin on cardiac angiogenesis, in old male rats.
MATERIALS AND METHODS
Rats randomly divided into young, age (rats of 26-28 months of age) and curcumin-age (rats of 26-28 months of age treatment with curcumin 50mg/kg). Finally, the expression of VEGF, NF-κB, and TSP-1 were assessed by ELISA in cardiac tissue. Also, angiogenesis was determined by immunostaining for PECAM-1/CD31 and apoptosis was evaluated by TUNEL.
RESULTS
After 2 months, curcumin-age had significantly higher cardiac VEGF-A and NF-κB and lower cardiac TSP-1 expression levels in comparison with age and young. A significant increase in levels of NF-κB and TSP-1 were observed in the age group.
CONCLUSION
Results suggest that curcumin through regulation of cardiogenic mediators and improving cardiac angiogenesis can promote heart performance in the senescent rats.
背景
心脏衰老属于不可逆转的过程，是由心脏形态和生理特性的多种缓慢进行性损伤变化所决定的。本研究旨在探讨姜黄素对老年雄性大鼠心脏血管生成的影响。
材料与方法
将大鼠随机分为青年组、老年组（26-28 月龄大鼠）和姜黄素-老年组（26-28 月龄大鼠给予姜黄素 50mg/kg 治疗）。最后，通过 ELISA 检测心脏组织中 VEGF、NF-κB 和 TSP-1 的表达，通过免疫组化检测 PECAM-1/CD31 评估血管生成，通过 TUNEL 评估细胞凋亡。
结果
2 个月后，与老年组和青年组相比，姜黄素-老年组心脏 VEGF-A、NF-κB 表达水平显著升高，心脏 TSP-1 表达水平显著降低。老年组 NF-κB 和 TSP-1 水平显著升高。
结论
结果表明，姜黄素通过调节心脏生成介质和改善心脏血管生成，可促进衰老大鼠的心脏功能。
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