Shargh Zahra, Asghari Keyvan, Asghariehahari Morteza, Chodari Leila
Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran.
Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran.
Heliyon. 2025 Jan 11;11(2):e41895. doi: 10.1016/j.heliyon.2025.e41895. eCollection 2025 Jan 30.
Inflammation serves as a key contributor to various diseases, necessitating the discovery of new treatment approaches to address its causative role.
The study involved 35 male Wistar rats, including 7 young rats (3-month-old; 200-250 g) in the Young control group and 28 aged rats (18-month-old; 400-450 g) randomly distributed among the Old control, Exercise, Curcumin, and Exercise + Curcumin groups. During an 8-week period, the Exercise group underwent running on the treadmill (17 m/min), while those in the Curcumin group were supplied with daily curcumin doses (50 mg/kg) through gavage. Upon completion of the study, serum samples from each group were collected for evaluating interleukin-6 (IL-6), interleukin-1β (IL-1β), interleukin-10 (IL-10), and Tumor Necrosis Factor-α (TNF-α) levels using ELISA; malondialdehyde (MDA) by enzymatic assay; and miR-21 and miR-146a by RT-PCR.
Our findings revealed that the Old control group, in contrast to the Young control group, showed a significant reduction in IL-10 serum levels, while MDA, TNF-α, IL-1β, and IL-6 serum levels were significantly elevated. Additionally, the expression of inflammatory microRNAs (miRNAs), miR-21 and miR-146a, was significantly enhanced in the Old control rats compared with the Young control group. Exercise and curcumin treatment alone resulted in an improvement in the expression of the markers and miRNAs associated with inflammation. Furthermore, when exercise and curcumin were administered simultaneously, a synergistic effect was observed compared to the exercise or curcumin alone groups.
Curcumin and exercise, individually and synergistically in combination, effectively reduced inflammation in aged rats, likely due to decreased oxidative stress and MDA levels mediated by miR-21 and miR-146a downregulation.
炎症是多种疾病的关键促成因素，因此需要发现新的治疗方法来解决其致病作用。
该研究涉及35只雄性Wistar大鼠，包括7只年轻大鼠（3个月大；200 - 250克）作为年轻对照组，以及28只老年大鼠（18个月大；400 - 450克），随机分布在老年对照组、运动组、姜黄素组和运动 + 姜黄素组。在8周期间，运动组在跑步机上跑步（17米/分钟），而姜黄素组每天通过灌胃给予姜黄素剂量（50毫克/千克）。研究结束后，收集每组的血清样本，使用酶联免疫吸附测定法（ELISA）评估白细胞介素 - 6（IL - 6）、白细胞介素 - 1β（IL - 1β）、白细胞介素 - 10（IL - 10）和肿瘤坏死因子 - α（TNF - α）水平；通过酶促测定法检测丙二醛（MDA）；通过逆转录 - 聚合酶链反应（RT - PCR）检测miR - 21和miR - 146a。
我们的研究结果显示，与年轻对照组相比，老年对照组的IL - 10血清水平显著降低，而MDA、TNF - α、IL - 1β和IL - 6血清水平显著升高。此外，与年轻对照组相比，老年对照大鼠中炎症微RNA（miRNA）miR - 21和miR - 146a的表达显著增强。单独的运动和姜黄素治疗导致与炎症相关的标志物和miRNA的表达有所改善。此外，与单独运动或姜黄素组相比，同时进行运动和姜黄素治疗时观察到协同效应。
姜黄素和运动单独或联合使用均能有效减轻老年大鼠的炎症，这可能是由于miR - 21和miR - 146a下调介导的氧化应激和MDA水平降低所致。
