Centre for Stem Cells and Regenerative Medicine, Faculty of Life Sciences & Medicine, King's College London, London, UK.
Instituto Maimonides de Investigacion Biomedica de Cordoba (IMIBIC), Cordoba, Spain; Departamento de Biologia Celular, Fisiologia e Inmunologia, Universidad de Cordoba, Cordoba, Spain; Hospital Universitario Reina Sofia, Cordoba, Spain.
Mol Cell Endocrinol. 2021 Dec 1;538:111459. doi: 10.1016/j.mce.2021.111459. Epub 2021 Sep 20.
Though embryonic pancreas progenitors are well characterised, the existence of stem/progenitor cells in the postnatal mammalian pancreas has been long debated, mainly due to contradicting results on regeneration after injury or disease in mice. Despite these controversies, sequencing advancements combined with lineage tracing and organoid technologies indicate that homeostatic and trigger-induced regenerative responses in mice could occur. The presence of putative progenitor cells in the adult pancreas has been proposed during homeostasis and upon different stress challenges such as inflammation, tissue damage and oncogenic stress. More recently, single cell transcriptomics has revealed a remarkable heterogeneity in all pancreas cell types, with some cells showing the signature of potential progenitors. In this review we provide an overview on embryonic and putative adult pancreas progenitors in homeostasis and disease, with special emphasis on in vitro culture systems and scRNA-seq technology as tools to address the progenitor nature of different pancreatic cells.
尽管胚胎胰腺祖细胞的特征已经得到很好的描述，但在哺乳动物胰腺中是否存在干细胞/祖细胞一直存在争议，主要是因为在小鼠的损伤或疾病后再生方面存在相互矛盾的结果。尽管存在这些争议，但测序技术的进步，结合谱系追踪和类器官技术，表明小鼠的体内平衡和触发诱导的再生反应可能会发生。在体内平衡和不同的应激挑战（如炎症、组织损伤和致癌应激）期间，已经提出了在成年胰腺中存在假定的祖细胞。最近，单细胞转录组学揭示了所有胰腺细胞类型中惊人的异质性，其中一些细胞表现出潜在祖细胞的特征。在这篇综述中，我们概述了胚胎和假定的成年胰腺祖细胞在体内平衡和疾病中的作用，特别强调了体外培养系统和 scRNA-seq 技术作为解决不同胰腺细胞祖细胞性质的工具。
