de Oliveira A C, Sawazaki H E, Galeazzi M A
Facultade de Engenharia de Alimentos e Agrícola Universidade Estadual de Campinas, São Paulo, Brasil.
Arch Latinoam Nutr. 1987 Mar;37(1):88-100.
Extraction, fractionation and characterization by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of proteins from Carioca 80 beans (Phaseolus vulgaris) were performed at three pH values (2.5, 8.0 and 9.0). Extraction at pH 7.0 proved to be more efficient and, after dialysis, produced a better separation of the albumin and globulin fractions. Relative mobility of the main protein in the globulin fractions occurred between 0.30 and 0.40, and dissociation was observed when the pH was increased. The two most representative bands gave molecular weights of 35,400 and 76,900, while in regard to the trypsin inhibitor, three bands gave 28,800, 22,500 and 18,300. Pepsin and pancreatin in vitro digestibility rendered values of 33.43% and 62.63% for whole flour and for protein precipitated at pH 4.5, respectively. The content of available methionine found, of 1.36 g/16 g N, appears to be high in relation to that of other bean varieties.
在三个pH值（2.5、8.0和9.0）下，对卡里奥卡80豆（菜豆）中的蛋白质进行了十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳提取、分级分离和表征。结果表明，在pH 7.0下提取效率更高，透析后，白蛋白和球蛋白组分的分离效果更好。球蛋白组分中主要蛋白质的相对迁移率在0.30至0.40之间，当pH值升高时会观察到解离现象。两条最具代表性的条带给出的分子量分别为35,400和76,900，而对于胰蛋白酶抑制剂，三条条带给出的分子量为28,800、22,500和18,300。全粉的胃蛋白酶和胰酶体外消化率分别为33.43%，在pH 4.5下沉淀的蛋白质的体外消化率为62.63%。所测得的可利用蛋氨酸含量为1.36 g/16 g N，相对于其他豆类品种而言似乎较高。
