Department of Bone Disease, The Second Hospital of Tangshan, No.21, Jianshe North Road, Tangshan, 063000, China.
Int J Clin Oncol. 2021 Dec;26(12):2347-2354. doi: 10.1007/s10147-021-02025-4. Epub 2021 Sep 21.
Osteosarcoma is the most common primary bone cancer in children and young adults. Recent experimental evidence has indicated that Runx2/OPN axis play important roles in the metastasis of osteosarcoma cells. The present study aimed to explore their relationship and prognostic significance in surgically resected osteosarcoma.
The expression of runt-related transcription factor2(Runx2) and osteopontin (OPN) in clinical specimens from 105 osteosarcoma patients were detected by immunohistochemistry. The correlations between Runx2, OPN, and clinicopathologic data were analyzed by Chi-square (χ) tests. The prognostic values were determined by univariate and multivariate survival analysis. The accuracy of oncologic outcome prediction was evaluated by receiver-operating characteristics curves.
The results showed there is a significant positive correlation between Runx2 and OPN expression at protein levels (P = 0.015). Runx2 and OPN were both independent predictors for overall survival and metastasis-free survival. When Runx2 and OPN were taken into consideration together, the predictive range was extended and the sensitivity was improved, and more significant and better biomarkers for osteosarcoma metastasis and survival.
These results suggest that a combined Runx2/OPN expression could be a valuable independent predictor of tumor metastasis and survival in osteosarcoma patients.
骨肉瘤是儿童和青少年中最常见的原发性骨癌。最近的实验证据表明，Runx2/OPN 轴在骨肉瘤细胞的转移中发挥重要作用。本研究旨在探讨其在手术切除的骨肉瘤中的关系和预后意义。
采用免疫组织化学法检测 105 例骨肉瘤患者临床标本中 runt 相关转录因子 2（Runx2）和骨桥蛋白（OPN）的表达。采用卡方检验（χ2 检验）分析 Runx2、OPN 与临床病理资料的相关性。采用单因素和多因素生存分析确定预后价值。通过受试者工作特征曲线评估肿瘤学结局预测的准确性。
结果表明，Runx2 和 OPN 在蛋白水平上的表达呈显著正相关（P = 0.015）。Runx2 和 OPN 都是总生存和无转移生存的独立预测因素。当同时考虑 Runx2 和 OPN 时，预测范围扩大，敏感性提高，是骨肉瘤转移和生存的更显著和更好的生物标志物。
这些结果表明，Runx2/OPN 表达的联合可以作为骨肉瘤患者肿瘤转移和生存的有价值的独立预测因子。
