Sorokin Dimitry Y, Yakimov Michail M, Messina Enzo, Merkel Alexander Y, Koenen Michel, Bale Nicole J, Sinninghe Damsté Jaap S
Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia; Department of Biotechnology, Section of Environmental Biotechnology, TU Delft, The Netherlands.
IAMC-CNR, Spianata S.Raineri 86, 98122 Messina, Italy.
Syst Appl Microbiol. 2021 Nov;44(6):126249. doi: 10.1016/j.syapm.2021.126249. Epub 2021 Sep 9.
Nine pure cultures of neutrophilic haloaloarchaea capable of anaerobic growth by carbohydrate-dependent sulfur respiration were isolated from hypersaline lakes in southwestern Siberia and southern Russia. According to phylogenomic analysis the isolates were closely related to each other and formed a new species within the genus Halapricum (family Haloarculaceae). They have three types of catabolism: fermentative, resulting in H formation; anaerobic respiration using sulfur compounds as e-acceptors and aerobic respiration. Apart from elemental sulfur, all isolates can also use three different sulfoxides as acceptors and the type strain also grows with thiosulfate, reducing it partially to sulfide and sulfite. All strains utilized sugars and glycerol as the e-donors and C source for anaerobic growth and some can also grow with alpha-glucans, such as starch and dextrins. The major respiratory menaquinones are MK-8:8 and MK-8:7, but 5-19% consists of "thermoplasmata" quinones (MMK-8:8 and MMK-8:7), whose occurrence in haloarchaea is unprecedented. On the basis of their unique physiological properties and results of phylogenomic analysis, the isolates are suggested to be classified into a novel species Halapricum desulfuricans sp. nov. (type strain HSR12-2 = JCM 34032 = UNIQEM U1001).
从西伯利亚西南部和俄罗斯南部的高盐湖中分离出了九株嗜中性嗜盐古菌纯培养物，它们能够通过依赖碳水化合物的硫呼吸进行厌氧生长。根据系统基因组分析，这些分离株彼此密切相关，并在嗜盐原菌属（嗜盐弧菌科）内形成了一个新物种。它们有三种分解代谢类型：发酵型，产生氢气；以硫化合物作为电子受体的厌氧呼吸以及有氧呼吸。除了元素硫外，所有分离株还可以使用三种不同的亚砜作为受体，模式菌株也能利用硫代硫酸盐生长，并将其部分还原为硫化物和亚硫酸盐。所有菌株都利用糖和甘油作为厌氧生长的电子供体和碳源，一些菌株还可以利用α-葡聚糖，如淀粉和糊精生长。主要的呼吸醌是MK-8:8和MK-8:7，但有5-19%是“嗜热栖热菌”醌（MMK-8:8和MMK-8:7），其在嗜盐古菌中的出现是前所未有的。基于它们独特的生理特性和系统基因组分析结果，建议将这些分离株归类为一个新物种——脱硫嗜盐原菌（Halapricum desulfuricans sp. nov.）（模式菌株HSR12-2 = JCM 34032 = UNIQEM U1001）。
