Department of Orthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, China; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China; Dental Department, Shanghai 1st People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.
Department of Orthodontics, Shanghai Stomatological Hospital, Fudan University, Shanghai, China; Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China.
Int Immunopharmacol. 2021 Nov;100:108134. doi: 10.1016/j.intimp.2021.108134. Epub 2021 Sep 20.
Transient receptor potential channel 6 (TRPC6) is a receptor-operated Ca channel that plays an important role in Ca influx in the majority of non-excitable cells and influences calcium signalling and cellular responses. Therefore, the purpose of the present study was to gain insight into the role of TRPC6 in the osteogenesis of periodontal ligament cells (PDLCs). By western blot and immunohistochemical staining, the protein level of TRPC6 was found to be increased in a time-dependent manner during osteoblastic differentiation of PDLCs. In addition, the TRPC6 inhibitor SKF96365 was used to block the function of TRPC6 and inhibit osteoblastic differentiation of PDLCs. The TRPC6 activator hyperforin dicyclohexylammonium salt (hyperforin DCHA) was used to activate TRPC6 and promote osteoblastic differentiation of PDLCs. In vivo, wild-type mice showed better bone regeneration than TRPC6 mice, suggesting that TRPC6 has notable osteogenic induction properties and is important for bone defect repair. In conclusion, the current data demonstrated that TRPC6 plays a significant role in osteoblastic differentiation of PDLCs, suggesting that it may be a promising therapeutic target in osteogenesis.
瞬时受体电位通道 6（TRPC6）是一种受体操纵的 Ca2+通道，在大多数非兴奋性细胞中，它在 Ca2+内流中起重要作用，并影响钙信号转导和细胞反应。因此，本研究旨在深入了解 TRPC6 在牙周膜细胞（PDLCs）成骨中的作用。通过 Western blot 和免疫组织化学染色，发现 TRPC6 的蛋白水平在 PDLCs 成骨分化过程中呈时间依赖性增加。此外，使用 TRPC6 抑制剂 SKF96365 阻断 TRPC6 的功能，抑制 PDLCs 的成骨分化。使用 TRPC6 激活剂贯叶连翘素二环己基铵盐（hyperforin DCHA）激活 TRPC6，促进 PDLCs 的成骨分化。在体内，野生型小鼠的骨再生能力优于 TRPC6 小鼠，表明 TRPC6 具有显著的成骨诱导特性，对骨缺损修复很重要。总之，目前的数据表明，TRPC6 在 PDLCs 的成骨分化中起重要作用，提示其可能成为成骨治疗的有前途的靶点。
