Kim Aeryun, Kim Ae Ri, Jeon Yeong-Eui, Yoo Yun-Jung, Yang Yu-Mi, Bak Eun-Jung
Department of Oral Biology, Yonsei University College of Dentistry, 134 Sinchon Dong, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Oral Health Research Institute, Apple Tree Dental Hospital, Bucheon, 14642, Republic of Korea.
Lab Anim Res. 2023 Sep 1;39(1):19. doi: 10.1186/s42826-023-00171-6.
Transient receptor potential canonical (TRPC) channels are non-selective cationic channels with permeability to Ca and Na. Despite their importance, there are currently few studies on TRPC in the periodontal ligament (PDL) and bone cells in the dental field. To provide biological information regarding TRPC in PDL cells and periodontal tissue, we evaluated TRPC channels expression in the osteoblast differentiation of PDL cells and periodontitis-induced tissue. Human PDL cells were cultured in osteogenic differentiation media for 28 days, and the expression of Runx2, osteocalcin (OCN), and TRPC1, 3, 4, and 6 was evaluated by real-time PCR. In ligature-induced periodontitis mice, the alveolar bone and osteoid areas, the osteoclast number, and the expression of Runx2, OCN, TRPC3, and TRPC6 was evaluated by H&E staining, TRAP staining, and immunohistochemistry, respectively.
In the PDL cell differentiation group, TRPC6 expression peaked on day 7 and TRPC3 expression generally increased during differentiation. During the 28 days of periodontitis progression, alveolar bone loss and osteoclast numbers increased compared to the control group during the experimental period and the osteoid area increased from day 14. TRPC6 expression in the periodontitis group increased in the PDL area and in the osteoblasts compared to the control group, whereas TRPC3 expression increased only in the PDL area on days 7 and 28.
These results indicate changes of TRPC3 and TRPC6 expression in PDL cells that were differentiating into osteoblasts and in periodontitis-induced tissue, suggesting the need for research on the role of TRPC in osteoblast differentiation or periodontitis progression.
瞬时受体电位香草酸亚型（TRPC）通道是对钙和钠具有通透性的非选择性阳离子通道。尽管其很重要，但目前在牙科领域中，关于牙周膜（PDL）和骨细胞中TRPC的研究较少。为了提供有关PDL细胞和牙周组织中TRPC的生物学信息，我们评估了PDL细胞成骨细胞分化和牙周炎诱导组织中TRPC通道的表达。将人PDL细胞在成骨分化培养基中培养28天，通过实时PCR评估Runx2、骨钙素（OCN）以及TRPC1、3、4和6的表达。在结扎诱导的牙周炎小鼠中，分别通过苏木精-伊红（H&E）染色、抗酒石酸酸性磷酸酶（TRAP）染色和免疫组织化学评估牙槽骨和类骨质面积、破骨细胞数量以及Runx2、OCN、TRPC3和TRPC6的表达。
在PDL细胞分化组中，TRPC6表达在第7天达到峰值，TRPC3表达在分化过程中总体上增加。在牙周炎进展的28天期间，与对照组相比，实验期间牙槽骨丢失和破骨细胞数量增加，类骨质面积从第14天开始增加。与对照组相比，牙周炎组中TRPC6在PDL区域和成骨细胞中的表达增加，而TRPC3仅在第7天和第28天在PDL区域表达增加。
这些结果表明，在分化为成骨细胞的PDL细胞和牙周炎诱导组织中，TRPC3和TRPC6表达发生了变化，这表明需要研究TRPC在成骨细胞分化或牙周炎进展中的作用。
