School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong Province, China.
Organic Reaction in Aqueous Engineering Research Center of Shandong Province, Chemical Technology Academy of Shandong Province, Jinan 250014, Shandong, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jan 15;265:120397. doi: 10.1016/j.saa.2021.120397. Epub 2021 Sep 14.
Sulfur dioxide (SO) is an important signal molecule in living systems, and plays a wide range of physiological functions. Real-time and in situ detection of the dynamic balance of SO in mitochondria is of great significance to in-depth study its biological roles. Herein, we have developed a mitochondria-targeted fluorescent probe Nap-L based on the FRET mechanism to detect SO in living cells. The probe Nap-L employed naphthalimide and positively charged benzopyridine as the donor and acceptor in the FRET system, and emitted green and red fluorescence under excitation. In respond to SO, the nucleophilic addition of bisulfite to benzopyridine and then interrupted the FRET process from naphthalimide to benzopyridine fluorophore, thereby triggering an obvious change in the fluorescence ratio. The probe Nap-L showed high selectivity to SO over the biothiols (Hcy, GSH, Cys) and other biologically related species. Biological experiments suggested that the probe Nap-L mainly distributed in mitochondria, and can be successfully used to detect mitochondrial endogenous SO in living cells.
二氧化硫（SO）是生命体系中的一种重要信号分子，具有广泛的生理功能。实时、原位检测线粒体中 SO 的动态平衡对于深入研究其生物学作用具有重要意义。在此，我们基于 FRET 机制开发了一种线粒体靶向荧光探针 Nap-L，用于检测活细胞中的 SO。探针 Nap-L 采用萘酰亚胺和带正电荷的苯并吡啶作为 FRET 系统中的供体和受体，在激发下发出绿色和红色荧光。响应 SO 时，亚硫酸氢根的亲核加成到苯并吡啶上，然后中断了从萘酰亚胺到苯并吡啶荧光团的 FRET 过程，从而引发荧光比明显变化。探针 Nap-L 对 SO 具有很高的选择性，超过了生物硫醇（Hcy、GSH、Cys）和其他相关生物物质。生物学实验表明，探针 Nap-L 主要分布在线粒体中，可成功用于检测活细胞中线粒体内源 SO。
