College of Modern Industry of Health Management, Jinzhou Medical University, Jinzhou 121001, China.
Molecules. 2024 Jan 23;29(3):554. doi: 10.3390/molecules29030554.
Monitoring the level of biothiols in organisms would be beneficial for health inspections. Recently, 3-(2'-nitro vinyl)-4-phenylselenyl coumarin as a fluorescent probe for distinguishing the detection of the small-molecule biothiols cysteine/homocysteine (Cys/Hcy) and glutathione (GSH) was developed. By introducing 4-phenyselenium as the active site, the probe CouSeNO/CouSNO was capable of detecting Cys/Hcy and GSH in dual fluorescence channels. Theoretical insights into the fluorescence sensing mechanism of the probe were provided in this work. The details of the electron excitation process in the probe and sensing products under optical excitation and the fluorescent character were analyzed using the quantum mechanical method. All these theoretical results would provide insight and pave the way for the molecular design of fluorescent probes for the detection of biothiols.
监测生物体内生物硫醇的水平将有益于健康检查。最近，3-(2'-硝基乙烯基)-4-苯基硒基香豆素作为一种荧光探针，用于区分小分子生物硫醇半胱氨酸/同型半胱氨酸（Cys/Hcy）和谷胱甘肽（GSH）的检测。通过引入 4-苯基硒作为活性位点，探针 CouSeNO/CouSNO 能够在双荧光通道中检测 Cys/Hcy 和 GSH。本工作提供了对探针荧光传感机制的理论见解。在光激发下，通过量子力学方法分析了探针和传感产物中电子激发过程以及荧光特性的详细信息。所有这些理论结果将为生物硫醇荧光探针的分子设计提供深入的了解和途径。
