Department of Ophthalmology, Fundação Altino Ventura, Recife, PE, Brazil.
Laboratory of Molecular Biology and Bacterial Genetics, Department of Tropical Medicine, Universidade Federal de Pernambuco, Recife, PE, Brazil.
Arq Bras Oftalmol. 2021 Sep 20;84(5):449-453. doi: 10.5935/0004-2749.20210070. eCollection 2021.
To analyze the presence of microorganisms in fluorescein eyedrops used in a reference eye center in Recife-PE.
This real-life and masked study evaluated fluorescein eyedrops used at the Altino Ventura Foundation in May 2019. Cultures were performed according to exposure times; I) three eyedrop bottles were analyzed after one day of use, II) three eyedrop bottles after 4 d of use, III) three eyedrop bottles after 8 d of use, and IV) three unopened bottles used as control. Samples were collected from the bottle's tip, instilled drop, and residual fluid. After incubation, all colonies were analyzed and identified through biochemical tests.
The contamination rate of the fluorescein eyedrop bottles in this study was 55.5% (5/9 vials). There was no contamination in the control group. The highest contamination was seen in one day exposed eyedrops, in 100% of the bottles. The bottle's tip had a higher rate of contamination compared to the drop and residual fluid. Gram-positive bacteria were isolated in 7/27 (25.9%) samples. Growth of fungi or gram-negative bacteria was not observed.
The identification of gram-positive bacteria predominantly on the tip of the fluorescein eyedrop bottles suggests inadequate handling as the main cause of contamination.
分析在累西腓-PE 的参考眼科中心使用的荧光素滴眼剂中存在的微生物。
本真实且掩蔽研究于 2019 年 5 月评估了在 Altino Ventura 基金会使用的荧光素滴眼剂。根据暴露时间进行培养；i）使用一天后分析三瓶滴眼剂，ii）使用四天后分析三瓶滴眼剂，iii）使用八天后分析三瓶滴眼剂，iv）使用三个未开封的瓶子作为对照。从瓶尖、滴注液和残留液采集样本。孵育后，通过生化试验分析和鉴定所有菌落。
本研究中荧光素滴眼剂瓶的污染率为 55.5%（9 瓶中有 5 瓶）。对照组没有污染。暴露一天的滴眼剂污染最严重，100%的瓶子都受到了污染。与滴注液和残留液相比，瓶尖的污染率更高。在 27 个样本中有 7 个（25.9%）分离出革兰氏阳性菌。未观察到真菌或革兰氏阴性菌的生长。
荧光素滴眼剂瓶瓶尖主要分离出革兰氏阳性菌，表明处理不当是污染的主要原因。
