Van Yüzüncü Yıl University, Faculty of Education, Department of Mathematics and Science Education, Van, Turkey.
Van Yüzüncü Yıl University, Science Faculty, Department of Molecular Biology and Genetic, Van, Turkey.
Braz J Biol. 2021 Sep 20;83:e251198. doi: 10.1590/1519-6984.251198. eCollection 2021.
The present study was designed to investigate the effects of Gundelia tournefortii L. plant extract on different tissues in terms of DNA damage, biochemical and antioxidant parameter values in rats with high-calorie diets. With this aim, Wistar albino male rats were divided into 4 groups containing 6 rats each and the study was completed over 12 weeks duration. At the end of the implementation process over the 12 weeks, rats were sacrificed and blood and tissue samples were obtained. Analyses were performed on blood and tissue samples. According to results for DNA damage (8-OHdG), in brain tissue the OG2 group was significantly reduced compared to the NC group. For MDA results in liver tissue, OG1 and OG2 groups were determined to increase by a significant degree compared to the control group, while the OG2 group was also increased significantly compared to the obese group. In terms of the other parameters, comparison between the groups linked to consumption of a high calorie diet (HCD) and administration of Gundelia tournefortii L. in terms of antioxidant activities and serum samples obtained statistically significant results. Gundelia tournefortii L. plant extracts had effects that may be counted as positive on antioxidant parameter activity and were especially identified to improve DNA damage and MDA levels in brain tissues. Additionally, consumption of Gundelia tournefortii L. plant extract in the diet may have antiobesity effects; thus, it should be evaluated for use as an effective weight-loss method and as a new therapeutic agent targeting obesity.
本研究旨在探讨厚藤（Gundelia tournefortii L.）植物提取物对高热量饮食大鼠不同组织中 DNA 损伤、生化和抗氧化参数值的影响。为此，将 Wistar 白化雄性大鼠分为 4 组，每组 6 只，研究持续 12 周。在 12 周的实施过程结束时，处死大鼠并获取血液和组织样本。对血液和组织样本进行了分析。根据 DNA 损伤（8-OHdG）的结果，脑组织中 OG2 组与 NC 组相比显著降低。对于肝组织中 MDA 的结果，OG1 和 OG2 组与对照组相比被确定为显著增加，而 OG2 组与肥胖组相比也显著增加。就其他参数而言，与高热量饮食（HCD）相关的组之间的比较以及在抗氧化活性和血清样本方面对厚藤植物提取物的给药进行了统计上的显著比较。厚藤植物提取物具有可能被视为对抗氧化参数活性有积极影响的作用，特别是被确定可以改善脑组织中的 DNA 损伤和 MDA 水平。此外，在饮食中摄入厚藤植物提取物可能具有抗肥胖作用；因此，应评估其作为一种有效的减肥方法和针对肥胖的新治疗剂的用途。
