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通过系统的延时成像揭示嗅觉回路组装的细胞基础。

Cellular bases of olfactory circuit assembly revealed by systematic time-lapse imaging.

机构信息

Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA 20417, USA.

出版信息

Cell. 2021 Sep 30;184(20):5107-5121.e14. doi: 10.1016/j.cell.2021.08.030. Epub 2021 Sep 21.

DOI:10.1016/j.cell.2021.08.030
PMID:34551316
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8545656/
Abstract

Neural circuit assembly features simultaneous targeting of numerous neuronal processes from constituent neuron types, yet the dynamics is poorly understood. Here, we use the Drosophila olfactory circuit to investigate dynamic cellular processes by which olfactory receptor neurons (ORNs) target axons precisely to specific glomeruli in the ipsi- and contralateral antennal lobes. Time-lapse imaging of individual axons from 30 ORN types revealed a rich diversity in extension speed, innervation timing, and ipsilateral branch locations and identified that ipsilateral targeting occurs via stabilization of transient interstitial branches. Fast imaging using adaptive optics-corrected lattice light-sheet microscopy showed that upon approaching target, many ORN types exhibiting "exploring branches" consisted of parallel microtubule-based terminal branches emanating from an F-actin-rich hub. Antennal nerve ablations uncovered essential roles for bilateral axons in contralateral target selection and for ORN axons to facilitate dendritic refinement of postsynaptic partner neurons. Altogether, these observations provide cellular bases for wiring specificity establishment.

摘要

神经回路组装具有从组成神经元类型同时靶向许多神经元过程的特点，但动力学仍了解甚少。在这里，我们使用果蝇嗅觉回路来研究通过嗅觉受体神经元 (ORN) 将轴突精确靶向到同侧和对侧触角叶中特定神经小球的动态细胞过程。对 30 种 ORN 类型的单个轴突进行延时成像，揭示了延伸速度、神经支配时间以及同侧分支位置的丰富多样性，并确定了同侧靶向是通过稳定短暂的间质分支来实现的。使用自适应光学校正的晶格光片显微镜进行快速成像表明，在接近目标时，许多表现出“探索分支”的 ORN 类型由从富含 F-肌动蛋白的中心发出的平行微管为基础的末端分支组成。触角神经消融揭示了双侧轴突在对侧目标选择中的重要作用，以及 ORN 轴突促进突触后靶神经元树突的细化。总的来说，这些观察结果为布线特异性的建立提供了细胞基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/e24aa92a7d91/nihms-1736620-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/ea460aae450e/nihms-1736620-f0002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/20d31045896b/nihms-1736620-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/d190986985d6/nihms-1736620-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/2caacfb4028c/nihms-1736620-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/5c2fb071b77e/nihms-1736620-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/e24aa92a7d91/nihms-1736620-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/ea460aae450e/nihms-1736620-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/b0b6cb85ca00/nihms-1736620-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/20d31045896b/nihms-1736620-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/d190986985d6/nihms-1736620-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/2caacfb4028c/nihms-1736620-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/5c2fb071b77e/nihms-1736620-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5edd/8545656/e24aa92a7d91/nihms-1736620-f0008.jpg

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