Xu Chuanyun, Li Zhuoran, Luo Liqun
Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA.
Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Biology Graduate Program, Stanford University, Stanford, CA 94305, USA.
STAR Protoc. 2025 Mar 21;6(1):103694. doi: 10.1016/j.xpro.2025.103694. Epub 2025 Mar 11.
Here, we present a protocol for cell-type-specific single-cell labeling and manipulation in Drosophila using a sparse driver system. We describe steps for generating constructs and fly lines, titrating heat-shocked durations for precise temporal control and desired sparsity, and co-expressing multiple transgenes for experiments. We demonstrate that this generalizable toolkit enables tunable sparsity, multi-color staining, single-cell trans-synaptic tracing, single-cell manipulation, and cell-autonomous gene function analysis. For complete details on the use and execution of this protocol, please refer to Xu et al..
在此，我们展示了一种使用稀疏驱动系统在果蝇中进行细胞类型特异性单细胞标记和操作的方案。我们描述了构建体和果蝇品系的生成步骤、滴定热休克持续时间以实现精确的时间控制和所需的稀疏性，以及为实验共表达多个转基因的步骤。我们证明，这个通用工具包能够实现可调稀疏性、多色染色、单细胞跨突触追踪、单细胞操作和细胞自主基因功能分析。有关此方案的使用和执行的完整详细信息，请参考Xu等人的研究。
