Retina Service, Angiogenesis Lab, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 243 Charles Street, Boston, MA, 02114, USA.
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, USA.
Sci Rep. 2021 Sep 22;11(1):18798. doi: 10.1038/s41598-021-97947-4.
To investigate local cell death differences in the attached and detached retina at different regions in a murine experimental retinal detachment model. Subretinal injection of sodium hyaluronate was performed in eight-week-old C57BL/6J mice. Retinal regions of interest were defined in relation to their distance from the peak of the retinal detachment, as follows: (1) attached central; (2) attached paracentral; (3) detached apex; and (4) detached base. At day 0, the outer nuclear layer cell count for the attached central, attached paracentral, detached apex, and detached base was 1247.60 ± 64.62, 1157.80 ± 163.33, 1264.00 ± 150.7, and 1013.80 ± 67.16 cells, respectively. There were significant differences between the detached base vs. attached central, and between detached base vs. detached apex at day 0. The detached apex region displayed a significant and progressive cell count reduction from day 0 to 14. In contrast, the detached base region did not show progressive retinal degeneration in this model. Moreover, only the detached apex region had a significant and progressive cell death rate compared to baseline. Immediate confounding changes with dramatic differences in cell death rates are present across regions of the detached retina. We speculate that mechanical and regional differences in the bullous detached retina can modify the rate of cell death in this model.
为了研究在鼠实验性视网膜脱离模型中不同脱离部位的附着和脱离视网膜的局部细胞死亡差异。在 8 周龄 C57BL/6J 小鼠中进行了玻璃酸钠的视网膜下注射。根据与视网膜脱离峰值的距离，将感兴趣的视网膜区域定义为：（1）附着中央；（2）附着旁中央；（3）脱离顶点；和（4）脱离基底。在第 0 天，附着中央、附着旁中央、脱离顶点和脱离基底的外核层细胞计数分别为 1247.60±64.62、1157.80±163.33、1264.00±150.7 和 1013.80±67.16 个细胞。脱离基底与附着中央之间以及脱离基底与脱离顶点之间在第 0 天存在显著差异。从第 0 天到第 14 天，脱离顶点区域的细胞计数显著且持续减少。相比之下，在这个模型中，脱离基底区域没有表现出进行性视网膜变性。此外，只有脱离顶点区域与基线相比具有显著且持续的细胞死亡率增加。脱离视网膜的不同区域存在明显差异的细胞死亡率急剧变化，这会立即产生混杂影响。我们推测，在疱性脱离视网膜中的机械和区域性差异可以改变这个模型中的细胞死亡速率。
