Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa, Turkey.
Andrologia. 2022 Feb;54(1):e14249. doi: 10.1111/and.14249. Epub 2021 Sep 22.
The aim of the present study was to evaluate the effects of an alpha-lipoic acid-supplemented extender on ram semen at a post-thaw stage and after incubation (6 hr). Semen samples were collected from five Kivircik Rams. Pooled semen was diluted with an egg yolk-based extender with different concentrations of alpha-lipoic acid (0.25 mmol L , 0.5 mmol L and 1 mmol L ) and without alpha-lipoic acid. Motility, plasma membrane functional integrity (HOST), acrosome integrity (FITC-Pisum sativum agglutinin) and DNA integrity (TUNEL) were assessed at post-thaw and 6 hr after incubation of the frozen-thawed semen. At the post-thaw stage, 0.25 mmol L alpha-lipoic acid had a positive effect on sperm motility and plasma membrane functional integrity compared to the control group (p < .05). At the post-incubation stage (6 hr), it was determined that the motility and plasma membrane functional integrity of the antioxidant groups were adversely affected compared to the control group (p < .05) and 1 mmol L dose of alpha-lipoic acid had a harmful effect on DNA integrity compared to the control group (p < .05). The results of the study demonstrated that alpha-lipoic acid has positive effects at post-thaw but have harmful effects on long term to ram semen.
本研究旨在评估在解冻后和孵育（6 小时）阶段，添加α-硫辛酸的稀释剂对公羊精液的影响。从 5 只基夫里克公羊中采集精液。将精液与不同浓度（0.25mmol/L、0.5mmol/L 和 1mmol/L）的含卵黄基础稀释剂或不含α-硫辛酸的稀释剂混合。在解冻后和冷冻解冻精液孵育 6 小时后，评估精子活力、质膜功能完整性（HOST）、顶体完整性（FITC-豌豆凝集素）和 DNA 完整性（TUNEL）。在解冻后阶段，与对照组相比，0.25mmol/Lα-硫辛酸对精子活力和质膜功能完整性有积极影响（p<.05）。在孵育后阶段（6 小时），与对照组相比，抗氧化剂组的精子活力和质膜功能完整性受到不利影响（p<.05），与对照组相比，1mmol/Lα-硫辛酸对 DNA 完整性有有害影响（p<.05）。研究结果表明，α-硫辛酸在解冻后有积极作用，但对公羊精液的长期影响是有害的。
