Bursa Uludağ Üniversitesi Veteriner Fakültesi, Dölerme ve Suni Tohumlama A.D., Görükle Kampüsü, Bursa, Turkey.
Bursa Uludağ Üniversitesi, Arıcılık Geliştirme-Uygulama ve Araştırma Merkezi-AGAM, Görükle Kampüsü, Bursa, Turkey.
Cryobiology. 2024 Mar;114:104849. doi: 10.1016/j.cryobiol.2024.104849. Epub 2024 Jan 17.
This study aimed to determine the effect of alpha-lipoic acid (ALA) on post-thaw quality of bee semen. In the study, semen from sexually mature drone were collected. A series of experiments were carried out in which the retrieved semen was diluted with diluents containing different ALA concentrations or without ALA supplement (control). Cryopreserved sperm were thawed, and evaluated for motility (phase-contrast microscope), plasma and acrosomal membrane integrity, mitochondrial membrane potential, and DNA fregmantation. The results obtained showed that the highest motility after thawing was observed in the groups containing ALA 0.25 mmol (P < 0.05). Likewise, plasma membrane integrity was found to be better preserved in the ALA 0.25 mmol-added group than in other groups. Acrosomal integrity were also higher in the ALA-containing groups than in the control group (P < 0.05). The results of this study show that ALA supplementation especially at 0.25 mmol improved post-thawed sperm motility, plasma membrane functionality, and mitochondrial membrane potantial quality of honeybee semen.
本研究旨在探讨α-硫辛酸（ALA）对蜜蜂精液解冻后质量的影响。在研究中，采集了性成熟雄蜂的精液。进行了一系列实验，其中取回的精液用含有不同 ALA 浓度的稀释剂或不含 ALA 补充剂（对照）稀释。冷冻保存的精子解冻后，通过相差显微镜评估其运动能力、质膜和顶体膜完整性、线粒体膜电位和 DNA 片段化。结果表明，在含有 0.25mmol ALA 的组中，解冻后精子的活力最高（P<0.05）。同样，添加 0.25mmol ALA 的组中质膜完整性比其他组更好。含 ALA 的组的顶体完整性也高于对照组（P<0.05）。本研究结果表明，AL A 补充剂，特别是 0.25mmol，可改善解冻后蜜蜂精液的精子活力、质膜功能和线粒体膜电位质量。
