Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged, 6726, Hungary.
Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, Szeged, 6726, Hungary.
Biol Futur. 2020 Jun;71(1-2):147-152. doi: 10.1007/s42977-020-00006-8. Epub 2020 May 13.
In this study, an in vitro α-chymotrypsin aggregation model was used to demonstrate that certain extracts of commercial coffees effectively inhibit protein aggregation in 55% ethanol at pH 7.0. To detect the anti-amyloidogenic effect of the various coffee extracts, turbidity measurements and Congo red binding assays were performed as well as the determination of the total polyphenol content of the extracts. The greatest fibril formation inhibitory effect was exerted by the Eduscho coffee extract, which contained also the most of the phenolic compounds. The Eduscho coffee extract inhibited the fibrillation of the α-chymotrypsin dose dependently. Coffee extracts are effective anti-aggregation agents, and their beneficial effects strongly correlate with the total phenolic content.
在这项研究中，使用体外α-糜蛋白酶聚集模型证明，某些商业咖啡提取物在 55%乙醇 pH7.0 下有效抑制蛋白质聚集。为了检测各种咖啡提取物的抗淀粉样蛋白形成作用，进行了浊度测量和刚果红结合试验以及提取物中总多酚含量的测定。Eduscho 咖啡提取物的纤维形成抑制效果最强，其所含的酚类化合物也最多。Eduscho 咖啡提取物剂量依赖性地抑制α-糜蛋白酶的纤维形成。咖啡提取物是有效的抗聚集剂，其有益效果与总酚含量密切相关。
