Shi Mingzi, Liu Chengguo, Zhao Yue, Wei Zimin, Zhao Maoyuan, Jia Liming, He Pingping
College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Instrumental Analysis Center, Northeast Agricultural University, Northeast Agricultural University, Harbin 150030, China.
Bioresour Technol. 2021 Dec;342:125901. doi: 10.1016/j.biortech.2021.125901. Epub 2021 Sep 8.
Denitrification during composting is a hidden danger that causes environmental pollution risk and aromatic humic substance damage, which needs to be better regulate urgently. In this study, two denitrification regulation methods, moisture and biochar amendment, were conducted during chicken manure composting. Denitrification performance data showed two regulation methods obviously reduced NO-N, NO-N and N contents. Humic substance increased by 25.3 % and 29.1 % under two regulations. Microbiological analysis indicated that two regulation methods could decreasing denitrifying functional microbes with aroma degradation capability. Subsequently, denitrification gene narG, nirS, nosZ were significantly inhibited (p < 0.05) and the aromatic degradation metabolism pathways were down-regulated. Correlation analysis further revealed the important influence of interspecific interactions and non-biological characteristics on functional microbes. These results provided important scientific basis to denitrification regulation in the practice of composting, which achieved the purpose of simultaneously controlling environmental pollution risk and conducing end-product formation.
堆肥过程中的反硝化作用是一个隐患，会导致环境污染风险和芳香族腐殖物质受损，急需更好地加以调控。在本研究中，在鸡粪堆肥过程中采用了两种反硝化调控方法，即调节水分和添加生物炭。反硝化性能数据表明，这两种调控方法显著降低了NO-N、NO-N和N的含量。在两种调控措施下，腐殖物质分别增加了25.3%和29.1%。微生物分析表明，这两种调控方法能够减少具有香气降解能力的反硝化功能微生物。随后，反硝化基因narG、nirS、nosZ受到显著抑制（p < 0.05），芳香族降解代谢途径下调。相关性分析进一步揭示了种间相互作用和非生物特性对功能微生物的重要影响。这些结果为堆肥实践中的反硝化调控提供了重要的科学依据，实现了同时控制环境污染风险和促进最终产物形成的目的。
