Department of First Operating Room, The First Hospital of Jilin University, Changchun 130021, China.
Department of Thoracic Surgery, The First Hospital of Jilin University, Changchun 130021, China.
Aging (Albany NY). 2021 Sep 23;13(18):22315-22331. doi: 10.18632/aging.203540.
Cathepsin like 2 (CTSL2) is a lysosomal cysteine protease, and may be associated with tumor metastasis. However, CTSL2 has not been reported as a biomarker in lung adenocarcinoma (LUAD). In this study, bioinformatics analysis using data from The Cancer Genome Atlas was performed. Wilcoxon rank-sum test and chi-square test were carried out. Kaplan-Meier and Cox regression were performed to evaluate the effect of CTSL2 expression in the overall survival. Our results indicated that CTSL2 in tumor was significantly higher than that in normal tissue ( < 0.001). High CTSL2 expression was significantly associated with age ( = 0.02), vital status (P < 0.001), and T classification ( = 0.03), and correlated with poor overall survival (HR = 1.62, 95% CI = 1.21-2.18, = 0.001). CTSL2 expression was an independent risk factor for overall survival in patients with LUAD (HR = 1.52, 95% CI = 1.12-2.05, = 0.006). A nomogram was plotted for illustration of CTSL2 expression on the risk of LUAD. Furthermore, cell experiments showed the CTSL2 promoted the proliferation and migration of A549 cells. In summary, high CTSL2 expression predicts poor prognosis in patients with LUAD.
组织蛋白酶样 2 (CTSL2) 是一种溶酶体半胱氨酸蛋白酶，可能与肿瘤转移有关。然而，CTSL2 尚未被报道为肺腺癌 (LUAD) 的生物标志物。在本研究中，使用来自癌症基因组图谱的数据进行了生物信息学分析。进行了 Wilcoxon 秩和检验和卡方检验。进行 Kaplan-Meier 和 Cox 回归分析以评估 CTSL2 表达对总生存期的影响。我们的结果表明，肿瘤中的 CTSL2 明显高于正常组织（<0.001）。高 CTSL2 表达与年龄（=0.02）、生存状态（P<0.001）和 T 分类（=0.03）显著相关，与总生存期不良相关（HR=1.62，95%CI=1.21-2.18，=0.001）。CTSL2 表达是 LUAD 患者总生存期的独立危险因素（HR=1.52，95%CI=1.12-2.05，=0.006）。绘制了列线图以说明 CTSL2 表达对 LUAD 风险的影响。此外，细胞实验表明 CTSL2 促进了 A549 细胞的增殖和迁移。总之，高 CTSL2 表达预示着 LUAD 患者预后不良。
