Department of Forest Resources and Wildlife Management, Faculty of Applied Science, National University of Science and Technology, P. O. Box AC 939, Ascot, Bulawayo, Zimbabwe.
Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.
BMC Complement Med Ther. 2021 Sep 23;21(1):238. doi: 10.1186/s12906-021-03407-x.
This study evaluated the in vitro antioxidant activity and comparison of anti-inflammatory and cytotoxic activity of Harpagopytum zeyheri with diclofenac.
In vitro assays were conducted using water, ethanol, and ethyl acetate extracts of H.zeyheri. The antioxidant activity was evaluated using the 2,2'-diphenyl-1-picrylhydrazy (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. The anti-inflammatory activity was determined by measuring the inhibition of nitric oxide (NO) on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages as well as cytokine (TNF-α and IL-10) expression on LPS-induced U937 human macrophages. For cytotoxicity, cell viability was determined using the 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
The ethyl acetate extract had the lowest IC values in the DPPH (5.91 μg/ml) and ABTS (20.5 μg/ml) assay compared to other extracts. Furthermore, the ethyl acetate extracts effectively inhibited NO and TNF-α and proved to be comparable to diclofenac at some concentrations. All extracts of H. zeyheri displayed dose-dependent activity and were associated with low levels of human-IL-10 expression compared to quercetin. Furthermore, all extracts displayed low toxicity relative to diclofenac.
These findings show that H. zeyheri has significant antioxidant activity. Additionally, similarities exist in the inflammatory activity of H. zeyheri to diclofenac at some concentrations as well as low toxicity in comparison to diclofenac.
本研究评估了 Harpagopytum zeyheri 水提物、醇提物和乙酸乙酯提取物的体外抗氧化活性，并与双氯芬酸进行了比较。
采用 2,2'-二苯基-1-苦基肼基（DPPH）和 2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法测定 Harpagopytum zeyheri 水提物、醇提物和乙酸乙酯提取物的体外抗氧化活性。通过测定脂多糖（LPS）诱导的 RAW 264.7 小鼠巨噬细胞一氧化氮（NO）的抑制作用以及 LPS 诱导的 U937 人巨噬细胞细胞因子（TNF-α和 IL-10）的表达，来评估其抗炎活性。采用 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法测定细胞活力来评价细胞毒性。
与其他提取物相比，乙酸乙酯提取物在 DPPH（5.91μg/ml）和 ABTS（20.5μg/ml）试验中具有最低的 IC 值。此外，乙酸乙酯提取物能有效抑制 NO 和 TNF-α，在某些浓度下与双氯芬酸相当。H. zeyheri 的所有提取物均显示出剂量依赖性活性，与槲皮素相比，其对人 IL-10 的表达水平较低。此外，与双氯芬酸相比，所有提取物的毒性均较低。
这些发现表明，H. zeyheri 具有显著的抗氧化活性。此外，在某些浓度下，H. zeyheri 的抗炎活性与双氯芬酸相似，与双氯芬酸相比，其毒性较低。
